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| Registros recuperados: 2.463 | |
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| Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena. |
| Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015 |
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| Miranda-Vilela,Ana L.; Akimoto,Arthur K.; Alves,Penha C.Z.; Pereira,Luiz C.S.; Klautau-Guimarães,Maria N.; Grisolia,Cesar K.. |
| Physical training induces beneficial adaptation, whereas exhaustive exercises increase reactive oxygen-species generation, thereby causing oxidative damage in plasma and erythrocytes, fractions susceptible to lipid peroxidation. Pequi (Caryocar brasiliense Camb.) is a Brazilian Cerrado fruit containing a carotenoid-rich oil. The aim was to investigate the effects of pequi-oil on exercise-induced oxidative damage in plasma and erythrocytes, after running in the same environment and undergoing weekly training under the same conditions as to type, intensity and length. Evaluations were accomplished after outdoor running on flat land before and after ingestion of 400 mg pequi-oil capsules for 14 days. Blood samples were taken after running and submitted to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caryocar brasiliense Camb hemogram hematimetric indexes (MCV; MCH; MCHC and RDW) haptoglobin antioxidant enzyme polymorphisms. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200026 |
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| Dematteis,Massimiliano. |
| Chromosomes of 12 Vernonia species (Asteraceae) from South America were studied. First counts were reported for: V. alpestris Gardn. (n = 17), V. chalybaea Mart. ex DC. (2n = 32), V. cotoneaster Less. (2n = 30), V. fruticulosa Mart. ex DC. (n = 16) and V. lithospermifolia Hieron. (n = 10, 2n = 20). Furthermore, in V. aurea Mart. ex DC. (2n = 32), V. mollissima D. Don (n = 32) and V. saltensis Hieron. (2n = 64) numbers different from those reported in the literature were found. Karyotypes were described for some species. V. aurea contains 24m + 8sm chromosomes, V. chalybaea contains 22m + 10sm chromosomes, V. cincta Griseb. 36m + 16sm + 8st and V. cotoneaster 18m + 12sm. The basic chromosome numbers x = 10, 14, 15, 16 and 17 were confirmed for South... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000300016 |
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| Bejarano,Diego; Martínez,Rodrigo; Manrique,Carlos; Parra,Luis Miguel; Rocha,Juan Felipe; Gómez,Yolanda; Abuabara,Yesid; Gallego,Jaime. |
| Abstract The linkage disequilibrium (LD) between molecular markers affects the accuracy of genome-wide association studies and genomic selection application. High-density genotyping platforms allow identifying the genotype of thousands of single nucleotide polymorphisms (SNPs) distributed throughout the animal genomes, which increases the resolution of LD evaluations. This study evaluated the distribution of minor allele frequencies (MAF) and the level of LD in the Colombian Creole cattle breeds Blanco Orejinegro (BON) and Romosinuano (ROMO) using a medium density SNP panel (BovineSNP50K_v2). The LD decay in these breeds was lower than those reported for other taurine breeds, achieving optimal LD values (r2 ≥ 0.3) up to a distance of 70 kb in BON and 100... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Creole breeds; BovineSNP50; Linkage disequilibrium; Minor allele frequency. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300426 |
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| Selivon,Denise; Perondini,André L.P.; Morgante,João S.. |
| Some aspects of reproductive isolation between allopatric populations of two closely related species of the Anastrepha fraterculus complex (A. fraterculus sp. 1 and sp. 2) were evaluated in laboratory conditions. Most of the crosses were fertile in each species as well as between sp. 2 females and sp. 1 males. In the reciprocal cross only 41.7% of the matings yielded viable progeny. Egg hatching occurred at similar rates within the two species, but was significantly lower in the crosses between the species. Adult emergence did not differ significantly among crosses. The sex ratio of adult progeny within each species, as well as in the hybrid progeny derived from sp. 1 females crossed to sp. 2 males, did not differ from the expected 1:1 ratio. However, in... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400007 |
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| Li,Xiaoyu; Chen,Wenjuan; Zhao,Yang; Xiang,Yan; Jiang,Haiyang; Zhu,Suwen; Cheng,Beijiu. |
| Lignin is a major cell wall component of vascular plants that provides mechanical strength and hydrophobicity to vascular vessels. However, the presence of lignin limits the effective use of crop straw in many agroindustrial processes. Here, we generated transgenic maize plants in which the expression of a lignin biosynthetic gene encoding CCoAOMT , a key enzyme involved in the lignin biosynthesis pathway was downregulated by RNA interference (RNAi). RNAi of CCoAOMT led to significantly downregulated expression of this gene in transgenic maize compared with WT plants. These transgenic plants exhibited a 22.4% decrease in Klason lignin content and a 23.3% increase in cellulose content compared with WT plants, which may reflect compensatory regulation of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CCoAOMT; Lignin biosynthesis; Lignin and cellulose content; Maize. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400013 |
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| Diniz Filho,José Alexandre Felizola; Villalobos,Fabricio; Bini,Luis Mauricio. |
| Eigenfunction analyses have been widely used to model patterns of autocorrelation in time, space and phylogeny. In a phylogenetic context, Diniz-Filho et al. (1998) proposed what they called Phylogenetic Eigenvector Regression (PVR), in which pairwise phylogenetic distances among species are submitted to a Principal Coordinate Analysis, and eigenvectors are then used as explanatory variables in regression, correlation or ANOVAs. More recently, a new approach called Phylogenetic Eigenvector Mapping (PEM) was proposed, with the main advantage of explicitly incorporating a model-based warping in phylogenetic distance in which an Ornstein-Uhlenbeck (O-U) process is fitted to data before eigenvector extraction. Here we compared PVR and PEM in respect to... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Evolutionary models; Phylogenetic comparative methods; Phylogenetic imputation; Phylogenetic signal. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300396 |
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| Villela,Thais R.; Freire,Bruna L.; Braga,Nathalia T. P.; Arantes,Rodrigo R.; Funari,Mariana F. A.; Alexander,Jorge A L; Silva,Ivani N.. |
| Abstract Laron’s syndrome (LS) is a rare genetic disorder characterized by insensitivity to growth hormone (GH). Up to the present time, over 70 mutations of GH receptor (GHR) gene have been identified leading to GH/insulin-like growth factor type 1 (IGF1) signaling pathway defect. The number of LS patients worldwide is unknown, as many are probably undiagnosed. We report two sibs from a consanguineous family from Minas Gerais, southeastern Brazil. The parents have three children. The older, a 4-years-old girl was 80.2 cm tall (-5.7 SDS height/age), and the youngest sister, aged 3 years, was 73.2 cm tall (-5.82 SDS height/age). Their clinical and biochemical features are typical of LS patients, such as high serum level of GH and low IGF1 concentrations. A... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laron Syndrome; Growth hormone; Growth hormone receptor; Genetics. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500102 |
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| Curi,Rogério A.; Palmieri,Darío A.; Suguisawa,Liliane; Oliveira,Henrique N. de; Silveira,Antonio C.; Lopes,Catalina R.. |
| The objectives of the present study were to estimate the allele and genotype frequencies of the GH1/Alu I and POU1F1/Hinf I polymorphisms in beef cattle belonging to different genetic groups and to determine the effects of these polymorphisms on growth and carcass traits in cattle submitted to feedlot management, an intensive production model. Genotyping was performed on 384 animals, including 79 Nellore, 30 Canchim (5/8 Charolais + 3/8 Zebu), 30 Simmental x Nellore crossbred and 245 Angus x Nellore crossbred cattle. Body weight, weight gain, dressing percentage, Longissimus dorsi area and backfat thickness were fitted using the General Linear Model (GLM) procedure of the SAS program and the least square means of the genotypes were compared using the F... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Beef cattle; Polymorphisms; Candidate gene; Growth; Carcass. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100012 |
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| Aral,Cenk; Akkiprik,Mustafa; Kaya,Handan; Ataizi-Çelikel,Çigdem; Çaglayan,Sinan; Özisik,Gökhan; Baloglu,Hüseyin; Özer,Ayse. |
| Recently, efforts have been focused on mitochondrial DNA changes and their relation to human cancers. Among them, a 4977 bp deletion of mitochondrial DNA, named "common deletion", has been investigated in several types of tumors, with inconsistent results. In this study, we investigated the presence of the common deletion in tissues from 25 breast, 25 colorectal and 50 thyroid tumors and in the adjacent healthy tissues from Turkish patients. Samples from healthy volunteers were also evaluated for comparison. Two PCR-based methods were used for the detection of the common deletion. First, two pairs of primers were used to amplify wild-type and deleted mtDNA. Then, a highly sensitive nested-PCR was performed, to determine low amounts of deleted genomes. By... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial DNA; Common deletion; Cancer. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100001 |
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| Kolovou,Genovefa; Kolovou,Vana; Ragia,Georgia; Mihas,Constantinos; Diakoumakou,Olga; Vasiliadis,Ioannis; Mavrogeni,Sophie; Vartela,Vassiliki; Manolopoulos,Vangelis G. |
| In this work, we examined the impact of polymorphism in the cytochrome P450 (CYP) 3A5 gene, CYP3A5*1 (6986A > G, rs 776746), on the reduction in the lipid levels caused by simvastatin and atorvastatin. We studied 350 hyperlipidemic patients who received 10-40 mg of atorvastatin (n = 175) or simvastatin (n = 175) daily. Genotyping for CYP3A5 was done by PCR-RFLP analysis. Differences in the lipid profile before and after treatment were expressed as the % difference. The frequency of CYP3A5 polymorphism was 13.4% for heterozygotes and 86.6% for homozygotes. Comparison of the responses to same dose of each drug showed that the highest % difference was associated with total cholesterol (TC) in subjects receiving atorvastatin 40 mg compared with simvastatin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atorvastatin; Cholesterol; CYP3A5 gene polymorphism; Simvastatin. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200129 |
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| Wang,Nan; Shi,Haifeng; Yao,Qin; Zhou,Yang; Kang,Lequn; Chen,Huiqin; Chen,Keping. |
| Alcohol dehydrogenases (ADH) are a class of enzymes that catalyze the reversible oxidation of alcohols to corresponding aldehydes or ketones, by using either nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP), as coenzymes. In this study, a short-chain ADH gene was identified in Bombyx mori by 5'-RACE PCR. This is the first time the coding region of BmADH has been cloned, expressed, purified and then characterized. The cDNA fragment encoding the BmADH protein was amplified from a pool of silkworm cDNAs by PCR, and then cloned into E. coli expression vector pET-30a(+). The recombinant His-tagged BmADH protein was expressed in E. coli BL21 (DE3), and then purified by metal chelating affinity chromatography. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5'-RACE PCRADH; Enzymatic activity; Recombinant protein. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200013 |
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| Registros recuperados: 2.463 | |
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