|
|
|
| Registros recuperados: 2.533 | |
|
|
| Swetlana,Nagal; Jain,P. C.. |
| Feather waste is generated in large amounts as a by-product of commercial poultry processing. This residue is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Eight strains of Bacillus, isolated from decomposing feathers were tested for the hydrolysis of feather wastes in the laboratory. Among these strains, Bacillus cereus KB043 was the best feather degrading organism when grown on basal medium containing 1% hen feather as sole source of carbon and nitrogen. It caused 78.16 ± 0.4 % degradation with a significant release of soluble protein (1206.15 ± 14.7 µg mL-1) and cysteine (20.63 ± 0.4 µg mL-1) in the cultivation fluid. The strain also showed the highest level of keratinase activity (39.10 ± 0.4 U mL-1). These data... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus; Feather degradation; Poultry waste; Soluble protein; Keratinase. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100028 |
| |
|
|
| Aliabadi,Nasrin; Aminzadeh,Saeed; Karkhane,Ali Asghar; Haghbeen,Kamahldin. |
| Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biochemical characterization; Chitinase; Cohnella sp. A01; Isolation; Thermostable. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400931 |
| |
|
|
| Nascimento,Rodrigo Pires do; Alves Junior,Nelson; Coelho,Rosalie Reed Rodrigues. |
| Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptomyces malaysiensis; Brewer's spent grains; Corn steep liquor; Proteinases; Submerged fermentation. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400020 |
| |
|
|
| Gaitán-Hernández,Rigoberto; Esqueda,Martín; Gutiérrez,Aldo; Beltrán-García,Miguel. |
| The chemical changes in barley-straw (BS), wheat-straw (WS) and vineyard-pruning (VP) substrates were determined during colonization of Lentinula edodes mycelia (during primordium development) in solid state fermentation. Primordia appeared 39-50 days after inoculation. VP appeared to promote early sporophore initiation. The concentration of hemicellulose in BS and VP decreased gradually from 25.5% to 15.6% and from 15.8% to 12.3%, respectively. However in WS, hemicellulose decreased from 27.2% to 9.5%. Lignin broke down continuously in BS and WS, with 31.8% and 34.4% degradation, respectively; higher than that of cellulose. During the pinning stage, the C:N ratio decreased in VP and BS, but not in WS. On all substrates the phenols decreased notably... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agricultural residues; Vegetative growth; Chemical properties; Shiitake mushroom. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100004 |
| |
|
| |
|
|
| Cleff,Marlete Brum; Meinerz,Ana Raquel; Xavier,Melissa; Schuch,Luiz Filipe; Meireles,Mário Carlos Araújo; Rodrigues,Maria Regina Alves; Mello,João Roberto Braga de. |
| The aim of this study was to evaluate the in vitro activity of the essential oil extracted from Origanum vulgare against sixteen Candida species isolates. Standard strains tested comprised C. albicans (ATCC strains 44858, 4053, 18804 and 3691), C. parapsilosis (ATCC 22019), C. krusei (ATCC 34135), C. lusitaniae (ATCC 34449) and C. dubliniensis (ATCC MY646). Six Candida albicans isolates from the vaginal mucous membrane of female dogs, one isolate from the cutaneous tegument of a dog and one isolate of a capuchin monkey were tested in parallel. A broth microdilution technique (CLSI) was used, and the inoculum concentration was adjusted to 5 x 10(6) CFU mL-1. The essential oil was obtained by hydrodistillation in a Clevenger apparatus and analyzed by gas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida; Origanum vulgare; Essential oil; In vitro. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100018 |
| |
|
|
| Oladipo,Oluwatosin Gbemisola; Awotoye,Olusegun Olufemi; Olayinka,Akinyemi; Bezuidenhout,Cornelius Carlos; Maboeta,Mark Steve. |
| ABSTRACT Increased environmental pollution has necessitated the need for eco-friendly clean-up strategies. Filamentous fungal species from gold and gemstone mine site soils were isolated, identified and assessed for their tolerance to varied heavy metal concentrations of cadmium (Cd), copper (Cu), lead (Pb), arsenic (As) and iron (Fe). The identities of the fungal strains were determined based on the internal transcribed spacer 1 and 2 (ITS 1 and ITS 2) regions. Mycelia growth of the fungal strains were subjected to a range of (0-100 Cd), (0-1000 Cu), (0-400 Pb), (0-500 As) and (0-800 Fe) concentrations (mgkg-1) incorporated into malt extract agar (MEA) in triplicates. Fungal radial growths were recorded every three days over a 13-days' incubation period.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungi metal tolerance; Mycoremediation; Heavy metal; Mine site; Eco-friendly clean-up strategy. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100029 |
| |
|
|
| Erzinger,Gilmar Sidnei; Silveira,Mauricio Moura da; Costa,José Paulo Castilho Lopes da; Vitolo,Michele; Jonas,Rainer. |
| Previously grown cells of the ethanologenic bacterium Zymomonas mobilis produce sorbitol and gluconic acid, in reactions catalysed by the periplasmic enzymes glucose-fructose oxidoreductase (GFOR) and glucono-delta-lactonase (GL). The GFOR/GL system activity, in cells to be used in this bioconversion, depends on growth conditions. In batch runs, with initial glucose concentrations (S0) from 42 to 230 g/L, the highest specific and total GFOR/GL activities were obtained with S0 = 153 g/L (12.6 U/g cells and 62 U/L). Higher S0 led to decreasing activities in fresh cells. With S0 = 209 g/L, the final specific activity was only 7.0 U/g. After disruption of cells, however, an activity over 15 U/g was revealed. Since growth inhibition with S0 over 153 g/L was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zymomonas mobilis; Glucose-fructose oxidoreductase; Glucose. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400008 |
| |
|
|
| Ferrari,Rafaela; Galiana,Antonio; Cremades,Rosa; Rodríguez,Juan Carlos; Magnani,Marciane; Tognim,M.C.B.; Oliveira,Tereza C.R.M.; Royo,Gloria. |
| The objective of this study was to identify mutations in the Quinolone Resistance Determining sources Regions (QRDR) of the gyrA, gyrB, parC, and parE genes and to determine if any of the qnr variants or the aac(6')-Ib-cr variant were present in strains of Salmonella spp. isolated in Brazil. A total of 126 Salmonella spp. strains from epidemic (n = 114) and poultry (n = 12) origin were evaluated. One hundred and twelve strains (88.8%) were resistant to nalidixic acid (NAL) and 29 (23.01%) showed a reduced susceptibility to ciprofloxacin (Cip). The mutations identified were substitutions limited to the QRDR of the gyrA gene in the codons for Serine 83, Aspartate 87 and Alanine 131. The sensitivity to NAL seems to be a good phenotypic indication of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; PMQR; QRDR mutation; Quinolone; Ciprofloxacin. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200046 |
| |
|
| |
|
| |
|
| |
|
|
| Baldotto,Lílian Estrela Borges; Olivares,Fábio Lopes; Bressan-Smith,Ricardo. |
| The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ananas comosus; Plant-growth promoting bacteria; Microscopy. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100015 |
| |
|
|
| Aguiar,Daniel M.; Hagiwara,Mitika K.; Labruna,Marcelo B.. |
| An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ehrlichia canis; Dog; Molecular Characterization; Ehrlichiosis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014 |
| |
|
| |
|
|
| Reis,Cristiane Bianchi Loureiro dos; Morandini,Liziane Maria Barassuol; Bevilacqua,Caroline Borges; Bublitz,Fabricio; Ugalde,Gustavo; Mazutti,Marcio Antonio; Jacques,Rodrigo Josemar Seminoti. |
| Abstract Biosurfactants have many advantages over synthetic surfactants but have higher production costs. Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost. The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule. The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi. To optimize biosurfactant production, a Plackett–Burman design and a central composite rotational design were used. The variables evaluated were pH, incubation period,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Giberella fujikuroi Surface tension NMR spectroscopy α; Β-Trehalose Trehalolipids. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500185 |
| |
|
| |
|
|
| Cardoso,Martha Oliveira; Ribeiro,Aldemir Reginato; Santos,Luciana Ruschel dos; Pilotto,Fernando; Moraes,Hamilton L.S. de; Salle,Carlos Tadeu Pippi; Rocha,Silvio Luís da Silveira; Nascimento,Vladimir Pinheiro do. |
| Eighty Salmonella Enteritidis strains isolated from broiler carcasses between May 1995 and April 1996 in the State of Rio Grande do Sul, Brazil, were tested for antibiotic susceptibility using the disk diffusion method. Resistance to colistin, novobiocin, erythromycin and tetracycline was observed in 100% of the isolates. The strains showed intermediate resistance at different levels to kanamycin (1.25%), enrofloxacin (3.75%), neomycin (3.75%), fosfomycin (20%), sulphonamides (86.25%) and nitrofurantoin (90%). Resistance to ciprofloxacin, norfloxacin, gentamicin, polymyxin B, sulphametrim and sulphazotrim was not found. Since resistance to antibiotics especially those introduced in the last decades, was detected, it is recommended that their use must be... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Antibiotic resistance; Broiler carcasses; Salmonella Enteritidis. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300030 |
| |
|
| |
| Registros recuperados: 2.533 | |
|
|
|
