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| Registros recuperados: 57 | |
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| Pepin, Jean-francois; Riou, Antoine; Renault, Tristan. |
| Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region, which is present in two copies in the OsHV-1 genome and encodes two proteins of unknown function. The assay was applied successfully to rapid diagnosis (100 minutes)... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; PCR; Oyster; OsHV 1; Detection; Herpes virus 1. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3653.pdf |
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| Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter. |
| Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses... |
| Tipo: Text |
Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf |
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| Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan. |
| Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted... |
| Tipo: Text |
Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf |
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| David, R.; Treguier, Cathy; Montagnani, Caroline; Belliard, Corinne; Levy, Peva; Nedelec, Georges; Joufoques, Vaiana; Remoissenet, G.; Gueguen, Yannick; Cochennec-laureau, Nathalie. |
| Tipo: Text |
Palavras-chave: Betanodavirus; Detection; French Polynesia; Genotype; Platax orbicularis. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11380/7972.pdf |
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| Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M. |
| To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is... |
| Tipo: Text |
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf |
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| Varsamos, S.; Pepin, Jean-francois; Sauvage, Christopher; Renault, Tristan. |
| OsHV-1 was the first herpesvirus to be identified in an invertebrate host and is associated to mortalities in the Pacific oyster (Crassostrea gigas) and other economically important bivalve species. Infections due to OsHV-1 are frequently reported and cause high mortality rates in larvae and spat of C. gigas. Detection of this pathogen is usually performed by PCR and/or in situ hybridization. This presentation will focus on the development of a new molecular diagnostic tool for OsHV-1 (and OsHV-1 variant 1) detection: the mini-array. This detection system, which allows multiple pathogens detection, combines viral DNA amplification by PCR and a specific hybridisation on a nylon membrane, revealed by a colorimetric reaction (DNA chip). Multiplex PCR involves... |
| Tipo: Text |
Palavras-chave: Diagnostic; Crassotrea gigas; Oyster; Detection; Herpesviridae; OsHV 1. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3652.pdf |
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| Renault, Tristan; Arzul, Isabelle. |
| Crassostrea gigas is increasingly being cultured in a great number of countries from animaIs originally introduced from Japan, and is one of the most exploited bivalve species in the EU (FAO, 1989). Although no serious pathogen was detected previously in Japanese oysters in Europe, mortalities have been observed since the introduction of this bivalve species into the EU. Thus, oyster cultivation may be endangered by the occurrence of epizootics, especially virus diseases, which are considered one of the maj or risks to production. Indeed, mortalities have been described among different species of ostreids and are associated with the presence of viruses belonging to various families. The first description of a virus was reported in adult Eastern oyters,... |
| Tipo: Text |
Palavras-chave: Pathology; Virology; Herpes-like infection; Detection; Diagnosis; Herpesviridae; Oyster; Crassostrea gigas; Mortality. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00044/15536/12915.pdf |
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| Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. |
| Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... |
| Tipo: Text |
Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf |
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| Bosch, Albert; Sanchez, Gloria; Abbaszadegan, Morteza; Carducci, Annalaura; Guix, Susana; Le Guyader, Soizick; Netshikweta, Rembuluwani; Pinto, Rosa M.; Van Der Poel, Wim H. M.; Rutjes, Saskia; Sano, Daisuke; Taylor, Maureen B.; Van Zyl, Walda B.; Rodriguez-lazaro, David; Kovac, Katarina; Sellwood, Jane. |
| Potential ways to address the issues that relate to the techniques for analyzing food and environmental samples for the presence of enteric viruses are discussed. It is not the authors' remit to produce or recommend standard or reference methods but to address specific issues in the analytical procedures. Foods of primary importance are bivalve molluscs, particularly, oysters, clams, and mussels; salad crops such as lettuce, green onions and other greens; and soft fruits such as raspberries and strawberries. All types of water, not only drinking water but also recreational water (fresh, marine, and swimming pool), river water (irrigation water), raw and treated sewage are potential vehicles for virus transmission. Well over 100 different enteric viruses... |
| Tipo: Text |
Palavras-chave: Enteric viruses; Gastroenteritis; Hepatitis; Detection; Concentration. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14191/11465.pdf |
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| Walling, Emilie; Vourey, Elodie; Ansquer, Dominique; Beliaeff, Benoit; Goarant, C.. |
| Aims: A description of bacterial pathogens in shrimp ponds is necessary to understand their pathogenesis. Vibrio nigripulchritudo was shown to contain saprophytic and pathogenic strains among New Caledonian isolates. We established a method to map the development of V. nigripulchritudo in pond sediments at three different genetic levels: the species level, then at the pathogenic cluster level and finally at the plasmid level, present only in all highly pathogenic isolates. Methods and Results: PCR methods were applied to shrimp pond sediments both before and after a mortality outbreak. Using crude samples, the species V. nigripulchritudo is not detected at first (0/42 samples at day 56 post stocking) but appears frequently in the sediments after the... |
| Tipo: Text |
Palavras-chave: Detection; Diagnosis; Diseases; Ecology; PCR; Plasmids; Soil; Virulence. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00004/11484/8177.pdf |
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| Registros recuperados: 57 | |
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