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| Registros recuperados: 51 | |
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| Teodoro Pardo, Claudia Verónica de. |
| El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los... |
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Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/569 |
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| Teodoro Pardo, Claudia Verónica de. |
| El objetivo de este estudio fue analizar la variabilidad genética en diferentes especies del género Anastrepha (Anastrepha ludens, A. obliqua, A. serpentina y A. striata), estudiar el flujo genético en A. ludens entre diferentes poblaciones de México y de Guatemala mediante un análisis isoenzimático, cuantificar ADN del ganglio cerebral y glándulas salivales y explorar la hibridación genómica in situ (GISH) en cada una de las especies de moscas en condiciones de laboratorio. Se analizaron los sistemas isoenzimáticos 6PGDH, IDH, ME, MDH, GPI y GOT en las cuatro especies de moscas en condiciones de laboratorio y campo, y para el flujo genético sólo cuatro sistemas fueron estudiados. Se examinaron 30 individuos (15 hembras y 15 machos) de cada población. Los... |
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Palavras-chave: Anastrepha; Contenido de ADN; Citometría de flujo; Electroforesis; GISH; Isoenzimas; Flujo genético; Variabilidad genética; DNA content; Flow cytometry; Electrophoresis; Isozymes; Gene flow; Genetic variability; Doctorado; Genética. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/569 |
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| Rocha,Vanessa Alves Lima; Maeda,Roberto Nobuyuki; Santa Anna,Lidia Maria Melo; Pereira,Nei. |
| This work aimed at the production of cellulases from pretreated sugarcane bagasse by the filamentous fungus Trichoderma harzianum IOC 3844 and their application in the hydrolysis of this same substrate, for a future use in second-generation ethanol production. The production of cellulases was optimized, which resulted in high enzymatic activities after 42 hrs of process in an instrumented bioreactor (CMCase 27,017 U x L-1; FPase 1,225 U x L-1; and β-glucosidase 609 U x L-1). The enzymatic extract was concentrated by using a hollow fiber membrane filtration system. The concentrated extract was applied in the hydrolysis of pretreated sugarcane bagasse, after 28 hrs of enzymatic reaction, displaying a similar catalytic performance of that attained... |
| Tipo: Journal article |
Palavras-chave: Electrophoresis; Enzymatic hydrolysis; Second generation ethanol; Trichoderma harzianum. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500012 |
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| Guiller, A; Madec, L. |
| Electrophoretic analysis of eight polymorphic loci in 23 mixed or monospecific populations of Cepaea nemoralis and Cepaea hortensis indicates important genetic dissimilarities between Breton and Pyrenean areas. The concordance between groups of samples and various latitudinal or altitudinal zones suggests that the influence of climatic factors could be involved. Founder effects also seem to increase the differentiation in some populations; the lower genetic variability in three pyrenean samples probably submitted to frequent extinctions could illustrate this process. Nevertheless, divergences corresponding to plurispecific samples suppose the absence of interactions between the two species. |
| Tipo: Text |
Palavras-chave: Mollusca; Cepaea hortensis; Cepaea nemoralis; Climate; Electrophoresis; Natural populations; Genetics. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-1714.pdf |
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| Varenne, A; Gareil, P; Colliec-jouault, Sylvia; Daniel, R. |
| The interaction of proteins with polysaccharides represents a major and challenging topic in glycobiology, since such complexes mediate fundamental biological mechanisms. An affinity capillary electrophoresis method has been developed to evidence the complex formation and to determine the binding properties between an anticoagulant polysaccharide of marine origin, fucoidan, and a potential target protein, antithrombin. This method is a variant of zonal electrophoresis in the mobility shift format. A fixed amount of protein was injected into a capillary filled with a background electrolyte containing the polysaccharide in varying concentrations. The effective mobility data of the protein were processed according to classical linearization treatments to... |
| Tipo: Text |
Palavras-chave: Antithrombin; Fucoidan; Electrophoresis; Protein interaction; Polysaccharides. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2114.pdf |
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| Auffret, Mathieu; Diouris, M; Herry, A. |
| A polyclonal rabbit anti-human lysozyme (Dako) was applied on histological sections from bivalve mollusc and revealed with a mouse anti-rabbit IgG fluorescein conjugated antibody to localise cellular lysozyme in tissues where its activity had previously detected by enzymatic techniques. Labelling occurred in the cytoplasm of epithelial cells covering the body. This is in agreement with the intracellular distribution of this enzyme. In the Lucinid Lucinella divaricata gills, the cytoplasm of bacteriocytes was also labelled. This suggests that antibacterial activity occurred in these cells. Control sections indicate that fluorescence was a consequence of true primary antibody fixation. However, its specificity should be verified by electrophoretical... |
| Tipo: Text |
Palavras-chave: Bivalvia; Immunology; Antibodies; Electrophoresis; Histology. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-1723.pdf |
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| Erdeş,Efe; Doğan,Tuğba Somay; Coşar,İlhan; Danışman,Tarık; Kunt,Kadir Boğaç; Şeker,Tamay; Yücel,Meral; Özen,Can. |
| Background Scorpion venoms are rich bioactive peptide libraries that offer promising molecules that may lead to the discovery and development of new drugs.Leiurus abdullahbayrami produces one of the most potent venoms among Turkish scorpions that provokes severe symptoms in envenomated victims.Methods In the present study, the peptide profile of the venom was investigated by electrophoretic methods, size-exclusion and reversed-phase chromatography and mass spectroscopy. Cytotoxic and antimicrobial effects were evaluated on a breast cancer cell line (MCF-7) and various bacterial and fungal species.Results Proteins make up approximately half of the dry weight of L. abdullahbayrami crude venom. Microfluidic capillary electrophoresis indicated the presence of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpion venom; Toxin; Peptide; Leiurus abdullahbayrami; Microfluidic capillary; Electrophoresis; Peptidomics; Venomics; Cytotoxicity; Antimicrobial activity; Turkey. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200338 |
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| Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.. |
| Eighty-nine of 144 isolates of Clostridium perfringens obtained from 187 samples of 71 bovine in several Brazilian states were submitted to esterase electrophoresis for typing. Mobilites electrophoresis, as parameter, were settled down by isolates from ATCC pattern of types A, B, C, and D. Of the 89 isolates, 43 (48.3%) were characterized as electrophoretic type A, 20 (22.5%) as D, 18 (20.2%) as C, and 3 (3.4%) as B. Five (5.6%) isolates did not identity with any type. Similarly, esterase electrophoresis enabled the typing of 94.4 % of the isolates, demonstrating to be an appropriate method for animal sample analyses. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Esterase. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200009 |
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| Baldassi,L.; Barbosa,M.L.; Bach,E.E.; Iaria,S.T.. |
| Ten out of eighty-nine strains biochemically identified as Clostridium perfringens, isolated from bovine organs, were selected by their different results showed in toxigenicity test on mice. Those and the standard strains, ATCC types A, B, C, and D, had their virulence exalted through serial intramuscular inoculation into guinea pigs. Results showed that, for toxigenic strains (6), one or two passages were enough to cause exaltation, while for the atoxigenic (4), five or six inoculations were needed. Esterase electrophoresis of standard and isolated strains, with and without exaltation, was performed. Electrophoresis analysis permits the following conclusions: strains that do not show any clinical symptoms in mice, when exalted demonstrate decreased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium perfringens; Bovine; Electrophoresis; Virulence exaltation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300007 |
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| Pineda, S.E.; Hernandez, D.R.; Brunetti, N.E.; Jerez, B.. |
| Morphological characters of two loliginid squids, Loligo gahi and Loligo sanpaulensis, in the SW Atlantic region off Argentina were studied to obtain a diagnostic criterion for their identification. Emphasis was placed on the taxonomic characters suitable to identify individuals in the whole size range. Considering the uncertainty associated to the correct identification of juveniles, their taxonomic status was confirmed using electrophoretic studies. Discrimination between species based on morphological characters proved to be more effective than meristic counts. A discriminant function that allows an easy separation of the two species is presented. The function, composed of two indices: fin width mantle length (FW/ML)and gladius width mantle length... |
| Tipo: Journal Contribution |
Palavras-chave: Meristic counts; Morphometry; Electrophoresis; Electrophoresis; Http://aims.fao.org/aos/agrovoc/c_2523. |
| Ano: 2002 |
URL: http://hdl.handle.net/1834/1583 |
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| Marinkelle, C.J.. |
| Se discuten las condiciones electroforéticas. Usualmente fueron obtenidos resultados óptimos usando agar “Difco Noble” 0.9%; buffer pH 8.4; un potencial iónico en los compartimientos de electrodos de 0.10 y para la preparación del agar de 0.05; condiciones eléctricas 25-60 V. por cm.; una corriente aproximadamente 25 m. A; temperatura cerca de 4°C (suministrada por la evaporación de éter de petróleo de punto de ebullición de 40°C a 60°C) y un tiempo de electroforesis aproximadamente de 23 minutos. Se discute en detalle el método de la preparación de las comidas de sangre de los artrópodos y su aplicación a las láminas para estudio. Dos minutos después de comenzar el experimento electroforético fue aparente que la comida de sangre era adecuada para la... |
| Tipo: Journal Contribution |
Palavras-chave: Electrophoresis; Agar; Electrophoresis; Agar; Http://aims.fao.org/aos/agrovoc/c_2523; Http://aims.fao.org/aos/agrovoc/c_172. |
| Ano: 1969 |
URL: http://hdl.handle.net/1834/3319 |
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| Registros recuperados: 51 | |
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