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Effects of fermentation substrates and conservation methods on the viability and antimicrobial activity of Weissella confusa and its metabolites Electron. J. Biotechnol.
Serna-Cock,Liliana; Enríquez-Valencia,Cruz E; Jiménez-Obando,Eliana M; Campos-Gaona,Rómulo.
Lactic acid bacteria produce metabolites with antagonistic activity against other bacteria. However, growth conditions and conservation methods may reduce the viability and antimicrobial activity of lactic acid bacteria. This study evaluated the effects of fermentation substrate, lyophilization (freeze-drying) and refrigeration on the viability and antimicrobial activity of Weissella confusa strain and its metabolites against pathogens responsible for bovine mastitis. W. confusa strain was grown in MRS broth and milk supplemented with yeast extract and glucose (MYEG). The collected fractions were preserved by lyophilization or under refrigeration at 4ºC. Every seven days, the viability of W. confusa strain and the stability of its metabolites were...
Tipo: Journal article Palavras-chave: Conservation; Inhibition; Lactic acid bacteria; Lyophilization.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300006
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Guava Psidium guajava seed flour and dry Aspergillus niger mycelium as nitrogen sources for the production of biomass and antimicrobial compounds produced by Weissella confusa Electron. J. Biotechnol.
Serna-Cock,Liliana; Mera-Ayala,José Daniel; Angulo-López,Jorge Eduardo.
Background: The fermentation substrate efficiency of glucose supplemented with guava seed flour (GGSF) or glucose supplemented with dry Aspergillus niger mycelium (GANM) was evaluated during the production of biomass and antimicrobial compounds by the lactic acid bacteria Weissella confusa. Results: The fermentation substrate efficiency was measured by comparing the biomass formation, substrate consumption, substrate conversion, antimicrobial activity and product yield. The antimicrobial activity was measured against a commercial Staphylococcus aureus strain. The results were compared against fermentations performed in a commercial substrate (CS), the MRS (Man-Rogosa-Sharpe) substrate. The fermentations were performed discontinuously for 4 hrs at 100 rpm...
Tipo: Journal article Palavras-chave: Agroindustrial residue; Bacteriocin; Biomass; Inhibition.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600017
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Selection of psychotrophic bacteria active against spoilage and pathogenic micro-organisms relevant for seafood products ArchiMer
Matamoros, Sébastien; Pilet, Marie-france; Gigout, Frédérique; Prevost, Hervé; Leroi, Françoise.
In this study, 51 seafood products were screened to select psychrophilic inhibiting lactic acid bacteria. 5575 colonies were tested for inhibition against four target strains : Listeria monocytogenes, Staphylococcus xylosus, Pseudomonas sp. and Serratia liquefaciens. 456 colonies (8,2%) showed inhibition and 132 (28,9%) of them were picked up. 54 isolates growing at 15°C but not at 30°C were selected. Basic phenotypic characteristics (Gram, catalase and oxidase test) were then tested. Finally, 52 presumptive psychrophilic LAB strains were selected. The inhibition spectrum of these strains was enlarged to 14 spoiling or pathogenic target strains relevant for seafood products. The inhibition profiles were recorded, and clustering was made to separate eight...
Tipo: Text Palavras-chave: Biopreservation; Seafood products; Psychrophilic lactic acid bacteria; Inhibition.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/00002/11339/7888.pdf
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Selection and evaluation of seafood-borne psychrotrophic lactic acid bacteria as inhibitors of pathogenic and spoilage bacteria ArchiMer
Matamoros, Sebastien; Pilet, Marie-france; Gigout, Frederique; Prevost, Herve; Leroi, Francoise.
In this study, inhibitory psychrotrophic lactic acid bacteria were isolated and investigated for future use in biopreservation of seafood products. Screening of 5575 colonies isolated from various seafood products resulted in the selection of 132 colonies presenting inhibitory properties. Among them, 52 isolates had characteristics of LAB and showed growth at 15 °C but not at 30 °C. The inhibition spectrum of these 52 isolates against 14 target strains (Gram-positive and -negative) showed inhibition of typical seafood spoiling and pathogenic bacteria and enabled the formation of seven interesting clusters. Sequencing of the 16S rRNA gene of a representative isolate from each cluster identified three Leuconostoc gelidum, two Lactococcus piscium, one...
Tipo: Text Palavras-chave: Carnobacterium alterfunditum; Lactobacillus fuchuensis; Leuconostoc gelidum; Lactococcus piscium; Psychrotrophic; Inhibition; Seafood; Lactic acid bacteria.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6472.pdf
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Formaldehyde preservation for deferred measurements of alkaline phosphatase activities in marine samples ArchiMer
Labry, Claire; Urvoy, Marion.
Alkaline phosphatases are the main enzymes required by microorganisms to hydrolyse organic phosphorus into available phosphate in aquatic environments. The investigations of alkaline phosphatase activity (APA) usually generate numerous samples (size fractionation, Michaelis-Menten kinetics). Therefore, convenient and reliable preservation of incubated samples for a deferred analysis would be very useful when measurements cannot be performed right away. The APA of marine pond waters was measured using 4-Methylumbelliferyl phosphate (MUF-P) as the fluorogenic substrate modelling natural organic phosphorus compounds. Where typical inhibitors of other enzymatic activities, such as 1% sodium dodecyl sulfate, mercuric chloride, or buffered solutions of ammonium...
Tipo: Text Palavras-chave: Alkaline phosphatase activity; Formaldehyde; Inhibition; Enzymes; Aquatic studies; Deferred measurements.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00656/76787/78211.pdf
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Études des interactions entre la bactérie bioprotectrice Lactococcus piscium et Brochothrix thermosphacta et Listeria monocytogenes dans la crevette tropicale ArchiMer
Fall, Papa-abdoulaye.
The bioprotective potential of Lactococcus piscium CNCM I-4031 towards a specific spoilage organism, Brochothrix thermosphacta, was studied on tropical cooked peeled shrimp packed under modified atmosphere. In co-culture, the growth of this spoilage bacterium was inhibited by 4 log ufc/g by L. piscium and the sensory quality of the product was improved during 31 days. The inhibition could not be attributed to lactic acid or nutritional competition for amino acids. The growth modelling of L. piscium and B. thermosphacta was performed in different conditions of temperature, pH and NaCl concentrations. The optimal growth values of these two bacteria are quite similar, however L. piscium seemed to be more psychrotolerant than B. thermosphacta but twice less...
Tipo: Text Palavras-chave: Biopreservation; Inhibition; Modélisation; Lactococcus piscium; Flore d’altération; Analyse sensorielle; Produits de la mer; Co-cultures; Biopreservation; Inhibition; Modelling; Lactococcus piscium; Spoilage microflora; Sensory analysis; Seafood products; Co-cultures.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00050/16164/13649.pdf
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Protective Effect of a Non-Bacteriocinogenic Lactococcus piscium CNCM I-4031 Strain Against Listeria monocytogenes in Sterilized Tropical Cooked Peeled Shrimp ArchiMer
Fall, Papa-abdoulaye; Leroi, Francoise; Chevalier, Frederique; Guerin, Charline; Pilet, Marie-france.
The protective activity of a non-bacteriocinogenic Lactococcus piscium CNCM I-4031 strain against Listeria monocytogenes was investigated in tropical cooked peeled shrimp stored at 8°C in modified atmosphere packaging (50% N2-50% CO2). When inoculated alone (L. piscium 107 CFU g-1 and L. monocytogenes 104 CFU g-1), protective culture and target strain grew very well on shrimp reaching a maximum cell number of 109 CFU g-1 after 7 and 14 days, respectively. In the presence of L. piscium, growth of L. monocytogenes was totally prevented after 3 days of storage. The count was 3.4 log CFU g-1 lower than in the control after 10 days and until the end of storage (31 days). Using the Seafood Spoilage and Safety Predictor Software (http://sssp.dtuaqua.dk), it was...
Tipo: Text Palavras-chave: Inhibition; Biopreservation; Challenge-test; Lactic acid; Nutritional competition.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00005/11624/8281.pdf
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Enzymatic and biochemical characterization of Bungarus sindanus snake venom acetylcholinesterase J. Venom. Anim. Toxins incl. Trop. Dis.
Ahmed,M; Latif,N; Khan,RA; Ahmad,A; Rocha,JBT; Mazzanti,CM; Bagatini,MD; Morsch,VM; Schetinger,MRC.
This study analyses venom from the elapid krait snake Bungarus sindanus, which contains a high level of acetylcholinesterase (AChE) activity. The enzyme showed optimum activity at alkaline pH (8.5) and 45ºC. Krait venom AChE was inhibited by substrate. Inhibition was significantly reduced by using a high ionic strength buffer; low ionic strength buffer (10 mM PO4 pH 7.5) inhibited the enzyme by 1. 5mM AcSCh, while high ionic strength buffer (62 mM PO4 pH 7.5) inhibited it by 1 mM AcSCh. Venom acetylcholinesterase was also found to be thermally stable at 45ºC; it only lost 5% of its activity after incubation at 45ºC for 40 minutes. The Michaelis-Menten constant (Km) for acetylthiocholine iodide hydrolysis was found to be 0.068 mM. Krait venom...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acetylcholinesterase; Inhibition; Krait; Ionic strength; Acetylthiocholine iodide; Bungarus sindanus; Snake venom.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200014
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Inhibición de los contaminantes del tempeh de soya OceanDocs
Mejías, E.; Delgado, R.; Silveira, I.; Lafargue, D.; Cárdenas, E..
Se evaluó el efecto de diferentes especias o condimentos sobre la calidad microbiológica del tempeh y la posible acción inhibidora de sus contaminantes. El tempeh se obtuvo a partir de frijol de soya el cual se sometió a los procesos de cocción, escurrimiento y fermentación. A continuación se procedió a incorporar los condimentos ensayados en dos proporciones por separado a la soya fresca antes de su inoculación con la cepa R. oligosporus, se mezclaron bien y se sometieron al proceso de fermentación, el cual tuvo lugar en bandejas de poliestireno, incubadas a 30 ± 2 ºC por un período de 18 a 20 h hasta obtener un producto con las características típicas del tempeh. De éste se tomaron muestras para determinar contenido de humedad, conteo total de viables y...
Tipo: Journal Contribution Palavras-chave: Biological contamination; Chemical activity; Tempeh; Bacterial contamination; Enterobacteriaceae; Inhibition; Garlic; Anions; Spices.
Ano: 2008 URL: http://hdl.handle.net/1834/5010
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Phytotoxic Potential of the Crude Extract and Leaf Fractions of Machaerium hirtum on the Initial Growth of Euphorbia heterophylla And Ipomoea grandifolia Planta Daninha
XIMENEZ,G.R.; SANTIN,S.M.O.; IGNOATO,M.C.; SOUZA,L.A.; PASTORINI,L.H..
ABSTRACT: Allelopathy is the term used to define any process involving secondary metabolites produced by plants and microorganisms that influence growth and development of agrobiological systems. Currently, it is sought to find allelochemicals of interest and know how to apply them in bio-herbicides to combat weeds. In this study, the effects of the crude leaf extract and fractions of Machaerium hirtum (Vell.) Stellfeld were analyzed on Euphorbia heterophylla L. (wild poinsettia) and Ipomoea grandifolia (Dammer) O’Donell (morning glory), as well as the occurrence of morphoanatomical changes. For this, 0.04 g of the crude extract and fractions were solubilized and diluted (50 mL) to concentrations of 0.1, 0.2, 0.4, and 0.8 g L-1 (m/v). Initial growth tests...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inhibition; Allelopathy; Morphoanatomy; Weeds.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100215
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Differential suppression of rice weeds by allelopathic plant aqueous extracts Planta Daninha
Khaliq,A; Matloob,A; Khan,M.B; Tanveer,A.
Herbicidal potential of different plant aqueous extracts was evaluated against early seedling growth of rice weeds in pot studies. Plant aqueous extracts of sorghum (Sorghum bicolor), sunflower (Helianthus annuus), brassica (Brassica compestris), mulberry (Morris alba), eucalyptus (Eucalyptus camaldunensis), and winter cherry (Withania somnifera) at a spray volume of 18 L ha-1 each at the 2-4 leaf stage of rice weeds viz horse purslane (Trianthema portulacastrum) [broad-leaf], jungle rice (Echinochloa colona), and E. crus-galli (barnyard grass) [grasses] and purple nut sedge (Cyperus rotundus) and rice flat sedge (C. iria) [sedges]. The results showed significant interactive effects between plant aqueous extracts and the tested weed species for seedling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelopathy; Aqueous extracts; Seedling growth; Weeds; Inhibition.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582013000100003
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Analysis of Genotypic Variability in Avena spp. Regarding Allelopathic Potentiality Planta Daninha
CARRARO-LEMES,C.F.; SCHEFFER-BASSO,S.M.; DEUNER,C.C.; BERGHAHN,S.C.T..
ABSTRACT: The recognized allelopathic activity of oats as a soil-cover plant induces the study of the potential of their germplasm. The objective of this study is to verify if there is genotype variability in the Avena spp. with regard to allelopathic activity. Extracts of A. strigosa ‘Agro Quaraí’, ‘Agro Esteio’, ‘Embrapa139’, ‘AF 12104’, ‘AF 12109’, ‘AF 12202’, ‘AF 12209’, A. sativa ‘UPFPS Farroupilha’ and ‘AF 1345 Ucraniana,’ were prepared in three concentrations w/v (5 g, 10 g, and 20 g of dry mass 100 mL-1). The experiment was conducted in a germination chamber for 15 days, with lettuce (Lactuca sativa L.) as the recipient plant. There was complete inhibition of germination under 20% w/v extracts and partial inhibition at 10% w/v. Only 5% w/v observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avena sativa L.; Avena strigosa Schreb; Weed; Germination; Inhibition.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100297
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"In vitro" effect of fungicides on mycelial growth of bipolaris maydis Sci. Agrar.Paran. / SAP
Yamashita, O. M.; Kobayashi, L.; Cassetari Neto, D.; Santi, A.; Peres, W. M.; Carvalho, M. A. C.; Kappes, C..
Leaf spot is a disease provoked by the fungus Bipolaris maydis, that it causes great damages for annual crops, as the corn, the wheat and the  oats. Tests "in vitro" were accomplished seeking to evaluate the efficiency in the inhibition of the mycelial growth of that patogenic fungus through five fungicides: tetraconazol, tebuconazole, azoxystrobin + cyproconazole, trifloxystrobin + ropiconazole and trifloxystrobin + cyproconazole were ested fungicides. For the determination of the efficiency f the products, the daily growth of the fungus was valuated, being compared with the control. It was determined the percentage of growth inhibition. All of the treatments were effective in the inhibition of the growthof this fungus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungus; Inhibition; Disease; Chemical control.
Ano: 2010 URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/4270
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THE IMPORTANCE OF ANTIMICROBIAL COMPOUNDS PRODUCED BY BENEFICIAL BACTERIA ON THE BIOCONTROL OF PHYTOPATHOGENS Acta biol.Colomb.
CESA-LUNA,Catherine; BAEZ,Antonino; QUINTERO-HERNÁNDEZ,Verónica; CRUZ-ENRÍQUEZ,Joel DE LA; CASTAÑEDA-ANTONIO,Ma Dolores; MUÑOZ-ROJAS,Jesús.
ABSTRACT Bacteria produce antimicrobial compounds to compete for nutrients and space in a particular habitat. Antagonistic interactions can be evaluated by several methodologies including the double-layer agar and simultaneous inhibition assays. Among the well-known inhibitory substances produced by bacteria are the broad-spectrum antibiotics, organic acids, siderophores, antifungal, and bacteriocins. The most studied bacterial genera able to produce these inhibitory substances are Enterococcus, Lactococcus, Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia, and Burkholderia. Some beneficial bacteria can promote plant growth and degrade toxic compounds in the environment representing an attractive solution to diverse issues in agriculture and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Antibiotic; Competition; Inhibition; PGPR.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000100140
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Método de difusión lumínica aplicado a la capacidad inhibitoria para epitopes ABO en Ascaris lumbricoides ABCL
Ponce de León,Patricia; Foresto,Patricia; Valverde,Juana.
Experiencias previas demostraron la presencia de epitopes ABO en Ascaris lumbricoides. El objetivo de este trabajo fue determinar la capacidad inhibitoria del parásito para estos epitopes por cuantificación de la hemaglutinación aplicando el método de difusión lumínica por partículas en suspensión. Se seleccionaron 8 extractos parasitarios (EA) de pacientes grupo sanguíneo B y 4 de grupo A (con y sin epitopes ABO). Los EA fueron enfrentados a diluciones de antisuero monoclonal (anti A o anti B) y el antisuero excedente fue neutralizado con eritrocitos de isogrupo. Se midió la extinción óptica de la aglutinación resultante. Se graficó el porcentaje de aglutinación en función de la dilución del antisuero para calcular Título-50 (T-50). Se definió Poder...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascaris lumbricoides; Sistema de grupo sanguíneo ABO; Capacidad inhibitoria; Difusión lumínica por partículas en suspensión; Ascaris lumbricoides; Inhibition.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572005000400008
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Allelopathic potential of bark and leaves of Esenbeckia leiocarpa Engl. (Rutaceae) Acta Botanica
Souza,Flaviana Maluf; Gandolfi,Sergius; Perez,Sonia Cristina Juliano Gualtieri de Andrade; Rodrigues,Ricardo Ribeiro.
We investigated the inhibitory potential of aqueous extracts of bark and leaves of Esenbeckia leiocarpa Engl. on lettuce germination and early seedling growth. We compared the effects of four concentrations (100, 75, 50 and 25%) of each extract to water and polyethylene glycol (PEG 6000) solution controls for four replicates of 50 seeds for germination and four replicates of ten seedlings for seedling growth. The inhibitory effects of E. leiocarpa extracts on the percentage of germination and on the germination speed seemed to be more than simply an osmotic effect, except for the percentage of seeds germinated in bark extracts. When compared to water control, both bark and leaf extracts delayed germination, and leaf extracts also affected the percentage of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allelopathy; Germination; Growth; Inhibition; Plant-plant interaction.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000100016
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Use of wild yeasts as a biocontrol agent against toxigenic fungi and OTA production Agronomy
Souza, Mariana Lino; Passamani, Fabiana Reinis Franca; Ávila, Carla Luiza da Silva; Batista, Luis Roberto; Schwan, Rosane Freitas; Silva, Cristina Ferreira.
This study evaluated the antagonistic potential of 32 wild yeast isolates from coffee and cocoa bean fermentation. These yeasts were inoculated in co-cultivation with Aspergillus carbonarius (CCDCA 10608 and CCDCA 10408) and Aspergillus ochraceus (CCDCA 10612) isolated from grapes and coffee beans. The mycelial growth and ochratoxin A (OTA) production were evaluated, and the spores were counted after cultivation at 28°C for seven days. The yeasts presented higher inhibitory effects (53% in relation to the control) over the mycelial growth of the isolated A. ochraceus (CCDCA10612). Pichia anomala CCMA0148 and Saccharomyces cerevisiae CCMA0159 provided the greatest inhibition of the growth of all fungal strains. All Pichia species presented the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.02.04-4 antagonistic activity; Ochratoxin A; Aspergillus; Inhibition; Pichia sp.; Saccharomyces sp Fitosanidade Microbiologia Agricola.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32659
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Phytotoxic potential of Drimys brasiliensis Miers for use in weed control Agronomy
Anese, Simoni; Gualtieri, Sonia Cristina Juliano; Grisi, Patrícia Umeda; Jatobá, Luciana de Jesus; Arduin, Marcos.
This study aims to evaluate the phytotoxicity potential of leaf and root extracts of Drimys brasiliensis on the germination and seedling growth of Panicum maximum and Euphorbia heterophylla and its influence on metaxylem cell size in the seedling roots of the latter specie. The leaf and root extracts were fractionated by partition chromatography, and the hexane and ethyl acetate fractions obtained from each organ were evaluated at different concentrations for phytotoxic activity in several bioassays. In seedling growth tests, we compared the effects of these fractions with the herbicide oxyfluorfen. The hexane fraction of the root extracts showed a higher inhibitory potential on the germination and growth of weeds and reduced the average size of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agronomia - Fisiologia vegetal allelopathy; Inhibition; Germination; Early growth. Controle de plantas daninhas.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19476
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The anti-inflammatory activity of standard aqueous stem bark extract of Mangifera indica L. as evident in inhibition of Group IA sPLA2 Anais da ABC (AABC)
DHANANJAYA,BHADRAPURA LAKKAPPA; SHIVALINGAIAH,SUDHARSHAN.
ABSTRACT The standard aqueous stem bark extract is consumed as herbal drink and used in the pharmaceutical formulations to treat patients suffering from various disease conditions in Cuba. This study was carried out to evaluate the modulatory effect of standard aqueous bark extract of M. indica on Group IA sPLA2. M. indica extract, dose dependently inhibited the GIA sPLA2 (NN-XIa-PLA2) activity with an IC50 value 8.1 µg/ml. M. indica extract effectively inhibited the indirect hemolytic activity up to 98% at ~40 µg/ml concentration and at various concentrations (0-50 µg/ml), it dose dependently inhibited the edema formation. When examined as a function of increased substrate and calcium concentration, there was no relieve of inhibitory effect on the GIA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Secretory phospholipase A2; Anti-inflammatory; Pro-inflammatory; Inhibition; Drug; Plants.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100197
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Activation of group II metabotropic glutamate receptors blocks zinc release from hippocampal mossy fibers Biol. Res.
Matias,Carlos M; Dionísio,Jose C; Saggau,Peter; Quinta-Ferreira,Maria Emilia.
BACKGROUND: The hippocampal CA3 area contains large amounts of vesicular zinc in the mossy fiber terminals which is released during synaptic activity, depending on presynaptic calcium. Another characteristic of these synapses is the presynaptic localization of high concentrations of group II metabotropic glutamate receptors, specifically activated by DCG-IV. Previous work has shown that DCG-IV affects only mossy fiber-evoked responses but not the signals from associational-commissural afferents, blocking mossy fiber synaptic transmission. Since zinc is released from mossy fibers even for single stimuli and it is generally assumed to be co-released with glutamate, the aim of the work was to investigate the effect of DCG-IV on mossy fiber zinc signals....
Tipo: Journal article Palavras-chave: Zinc; Neurotransmission; Glutamatergic; Co-release; Metabotropic fluorescence; Inhibition.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100067
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