Data/hora: 05/06/2024 00:34:57

Provedor de dados: 	19	
País: 	Japan	
Título: 	In vitro inhibitory effect of fosmidomycin on the asexual growth of Babesia bovis and Babesia bigemina	
Autores: 	Sivakumar, T; Aboulaila, M. R. A.; Khukhuu, A; Iseki, H; Alhassan, A; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.	
Data: 	2008-12	
Ano: 	2008	
Palavras-chave: 	Fosmidomycin; Babesia bovis; Babesia bigemina; Asexual growth; In vitro culture.	
Resumo: 	The in vitro inhibitory effect of fosmidomycin was evaluated against the asexual growth of bovine Babesia parasites (Babesia bovis and Babesia bigemina). In the cultures with these parasites, severe reduction of parasite growth was observed by the addition of 1.25 μg/ml of the final drug concentration. Under these conditions, B. bigemina was completely cleared until the third day of cultivation. On the other hand, B. bovis was completely eliminated on the fourth day when the concentration was 6.25 μg/ml in the culture. These parasites failed to grow again when a drug-free medium was substituted for the subsequent cultivation. The IC50 values of fosmidomycin against B. bovis were determined as 0.88 and 0.63 μg/ml in the in vitro cultures with serum-containing M199 and serum-free GIT media, respectively, while it was 0.55 μg/ml against B. bigemina in a serum-containing M199 medium. Severe morphological changes, such as pycnotic and degenerative changes, were preferably observed in the treated parasites. These results suggest that fosmidomycin can be a potent chemotherapeutic agent against bovine babesiosis.	
Idioma: 	Inglês	
Identificador: 	http://ir.obihiro.ac.jp/dspace/handle/10322/2235	
Editor: 	National Research Center for Protozoan Diseases	
Formato: 	application/pdf	
Fonte: 	National Research Center for Protozoan Diseases