Data/hora: 03/06/2024 04:27:14

Provedor de dados: | 27
País: | Belgium
Título: | Simultaneous determination of a-tocopheryl acetate and tocopherols in aquatic organisms and fish feed
Autores: | Huo, J.-Z.; Nelis, H.; Lavens, P.; Sorgeloos, P.; De Leenheer, A.P.
Data: | 1999
Ano: | 1999
Palavras-chave: | Vitamin E; Vitamins.
Resumo: | In aquaculture, alpha-tocopheryl acetate (alpha-TA) is the main source of vitamin E used to fortify fish feed. Alpha-TA in fish is often determined indirectly, i.e. by alkaline hydrolysis, followed by quantitation of ‘total alpha-tocopherol’ (alpha-T) and substraction of the natively present alpha-T. The aim of this study was to develop an HPLC method for the simultaneous quantitative determination of alpha-TA and free tocopherols in aquatic organisms and fish feed. The assay consists of a simple extraction with methanol containing butylhydroxytoluene (BHT) as an antioxidant, followed by reversed-phase chromatography with consecutive UV and fluorescence detection of alpha-TA and tocopherols, respectively. The peak of the internal standard tocol in the fluorescence trace was used for quantitation. Linearity was achieved over the range of 0.2 to 4.2 µg alpha-TA per ml extract of &lt;i&gt;Artemia&lt;/i&gt; nauplii, which would correspond to 30.7 to 614.4 µg/g dry mass. The within-run coefficient of variation was 1.9% at a level of 310 µg/g dry mass. The recovery of alpha-TA ranged from 97.7 to 100.8% (concentration=2.1 and 20.5 µg/ml, &lt;i&gt;n&lt;/i&gt;=6). The detection limit was about 7 ng and the quantification limit on spiked samples was 0.2 µg/ml. This method was routinely applied to determine alpha-TA and alpha-, gamma- and delta-tocopherol (alpha-T, gamma-T, delta-T) simultaneously in &lt;i&gt;Artemia&lt;/i&gt; , fish feed, shrimp eggs and various other aquatic organisms.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.vliz.be/nl/open-marien-archief?module=ref&refid=4020
Fonte: | %3Ci%3EJ.+Chromatogr.+B%28724%29%3C%2Fi%3E%3A+249-255
Fonte: | info:eu-repo/semantics/restrictedAccess