Data/hora: 13/06/2024 02:51:05

Provedor de dados: | 3
País: | Brazil
Título: | Analysis of two drying methods on the yield and activity of sulfated polysaccharides extracted from Halymenia sp. (Rhodophyceae) - doi: 10.4025/actascibiolsci.v34i1.6961
Autores: | Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENÓRBIO; Vanderlei, Edfranck de Sousa Oliveira; Universidade Federal do Ceará - UFC; Quinderé, Ana Luíza Gomes; Universidade Federal do Ceará - UFC; Queiroz, Ismael Nilo Lino de; Universidade Federal do Ceará - UFC; Bessa, Érika Façanha; Universidade Federal do Ceará - UFC; Benevides, Norma Maria Barros; Universidade Federal do Ceará - UFC.
Data: | 2011-11-03
Ano: | 2011
Palavras-chave: | 2.08.01.00-9 Halymeniales; Sulfated macromolecules; Drying methods; Blood coagulation 2.08.01.00-9.
Resumo: | Sulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from Halymenia sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs) were first extracted with papain in 100 mM sodium acetate (pH 5.0) containing cysteine and EDTA (5 mM). The TSPs obtained were dried in an oven (M I) or lyophilized (M II) and then examined by ion exchange chromatography (DEAE-cellulose) using the NaCl gradient technique. The fractions were analyzed by 0.5% agarose gel electrophoresis and the in vitro anticoagulant activity was evaluated by the activated partial thromboplastin time test using normal human plasma and compared to heparin (HEP) (193.00 IU mg-1). There was a difference in TSP yield of 19.05% and similar chromatographic SP profiles. Electrophoresis revealed fractions with distinct resolutions. The fractions eluted with 0.75 M of salt (M I and II) were the most active, measuring 27.40 and 72.66 IU mg-1, respectively, when compared to HEP. Therefore, obtaining SP with anticoagulant activity from Halymenia sp. is more efficient by freeze-drying.
Idioma: | Português

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6961

10.4025/actascibiolsci.v34i1.6961

Editor: | Universidade Estadual De Maringá

Formato:

application/pdf

application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 34, n. 1 (2012); 5-11

Acta Scientiarum. Biological Sciences; v. 34, n. 1 (2012); 5-11

Fonte:

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