Data/hora: 03/06/2024 05:18:26

Provedor de dados: | 3
País: | Brazil
Título: | Analytical methods for evaluation of the fatty acid metabolism in rat liver
Autores: | Gilglioni, Eduardo Hideo; Campos-Shimada, Lilian Brites; Martins Maciel, Elismari Rizato; Ishii-Iwamoto, Emy Luiza; Salgueiro-Pagadigorria, Clairce Luzia.
Data: | 2018-11-30
Ano: | 2018
Palavras-chave: | 2.08.03.00-1 Metabolismo e Bioenergética liver; Fatty acid/metabolism; Fatty liver; Hepatic steatosis. Bioquímica.
Resumo: | The liver is an essential organ for body energy homeostasis, controlling the biosynthesis, uptake and the disposal of carbohydrates and lipids. The hepatic steatosis is a common condition frequently associated with metabolic diseases and is characterized by the excessive accumulation of triglycerides in the liver. In recent years, many efforts have been devoted to prevent and treat the hepatic steatosis, but it remains being pointed out as the major cause for chronic hepatic diseases in Western countries. A considerable part of the knowledge about the physiopathology of hepatic steatosis, the effects of diets and drugs on the metabolic capacity of the liver to metabolize fatty acids, as well as the potential therapeutic approaches for hepatic steatosis derived from experimental animal models using rodents. Here, in this article, we present the details of some of the most common techniques used to evaluate fatty acid metabolism in liver of rats, including quantification of total lipid content, measurement of fatty acid oxidation in isolated subcellular fractions and procedures to measure the activities of important lipogenic enzymes. Classical protocols previously described to be performed using samples from other tissues were adapted to liver samples and different techniques with equivalent aims were compared. The principles and the advantages in terms of reliability and costs were discussed and the procedures here described can be applied for a low-cost broad evaluation of the fatty acid metabolism in liver of rats submitted to different experimental conditions.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40040

10.4025/actascibiolsci.v40i1.40040

Editor: | Universidade Estadual De Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40040/pdf
Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e40040

Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e40040

1807-863X

1679-9283

Fonte:

Direitos autorais 2018 Acta Scientiarum. Biological Sciences

http://creativecommons.org/licenses/by/4.0