Data/hora: 13/06/2024 09:59:41

Provedor de dados: | 3
País: | Brazil
Título: | Molecular characteristics of polysulfated fractions from the green seaweed Caulerpa cupressoides and actions on thrombin generation in vitro
Autores: | Rodrigues, José Ariévilo Gurgel; Benevides, Norma Maria Barros; Tovar, Ana Maria Freire; Mourão, Paulo Antônio de Souza.
Data: | 2019-04-16
Ano: | 2019
Palavras-chave: | Caulerpaceae; Marine alga; Sulfated glycans; Thrombosis.
Resumo: | Caulerpa cupressoides produces sulfated polysaccharides (Cc-SPs) with serpin-dependent anticoagulant effect, but their actions on thrombin generation (TG) are unknown. This study aimed to partially characterize Cc-SPs and examine their potential as modulators of TG. Infrared analysis characterized extract containing three ulvan fractions (Cc-SP1, -SP2 and -SP3) separated by DEAE-cellulose chromatography, with differences in the relative proportions of sulfate (10.99-18.38%) and total sugars (46.59-51.12%), without presenting proteins. Charge density patterns and nonSPs varying from 8 to &gt; 100 kDa on agarose and polyacrylamide gel electrophoresis by sequential staining with toluidine blue and stains-all were also confirmed by gel permeation chromatography. The molecular weight of Cc-SP2 was not altered after treatment with 0.4 M HCl up to 5 h. Only Cc-SP2 altered the activated partial thromboplastin time (15 ± 0.3 IU) vs. heparin (193 IU) and abolished at high concentrations (&gt; 4.1 µg) TG by intrinsic pathway in 60-fold diluted human plasma, while at 4.1 µg attenuated TG by 33.87% delaying the lag phase (32 min.) vs. control (28 min.). Cc-SP2 induced concentration-dependent TG in system without cephalin. Heparin abolished TG at 4.15-fold lower amount, but did not stimulate TG. Therefore, Cc-SPs express dual effects on thrombosis in vitro.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/35655

10.4025/actascibiolsci.v41i1.35655

Editor: | Universidade Estadual De Maringá

Relação:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/35655/pdf

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/downloadSuppFile/35655/14384

Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 41 (2019): Publicação contínua; e35655

Acta Scientiarum. Biological Sciences; v. 41 (2019): Publicação contínua; e35655

1807-863X

1679-9283

Fonte:

Direitos autorais 2019 Acta Scientiarum. Biological Sciences

http://creativecommons.org/licenses/by/4.0