Data/hora: 20/05/2024 15:28:48

Provedor de dados: | 3
País: | Brazil

Título:

Anticoagulant properties of a crude sulfated polysaccharide from the red marine alga Halymenia floresia (Clemente) C. Agardh - doi: 10.4025/actascibiolsci.v33i3.6402

Anticoagulant properties of a crude sulfated polysaccharide from the red marine alga Halymenia floresia (Clemente) C. Agardh - doi: 10.4025/actascibiolsci.v33i3.6402

Autores: | Amorim, Rodrigo César das Neves; Universidade Federal da Amazônia; Rodrigues, José Ariévilo Gurgel; Rede Nordeste de Biotecnologia - RENORBIO; Holanda, Márjory Lima; Universidade Federal do Maranhão; Mourão, Paulo Antônio de Souza; Universidade Federal do Rio de Janeiro; Benevides, Norma Maria Barros; Universidade Federal do Ceará.
Data: | 2011-07-26
Ano: | 2011
Palavras-chave: | 2.08.01.00-9 Halymeniales; Halymenia floresia; Sulfated galactans; Anticoagulant activity 2.08.01.00-9 Halymeniales; Halymenia floresia; Sulfated galactans; Anticoagulant activity.
Resumo: | Alternative sources of anticoagulants have arisen as a result of the increasing demand for safer anticoagulant clinical therapy, and the sulfated polysaccharides of seaweeds have gained attention in biomedicine. In this study, crude sulfated polysaccharide fractions (denominated Hf1, Hf2 and Hf3) were obtained from the red marine alga Halymenia floresia and the anticoagulant properties of a soluble crude polysaccharide fraction (Hf2s) were assayed. The three differential extractions yielded 38.6%. The polysaccharides are composed mainly of galactose, with small amounts of xylose and glucose. The anticoagulant properties of Hf2s containing 53.8% sulfate and 3% protein was also compared to those of heparin (193.0 IU mg-1) by assays of activated partial thromboplastin time (APTT) and thrombin time (TT) using normal human plasma. Hf2s showed a higher anticoagulant activity (68.4 IU mg-1) than those of Hf1s and Hf3s, whose activities were 37.6 and 36.6 IU mg-1, respectively. The compound was less active than heparin, but its anticoagulant mechanism suggested that it is dependent on cofactor heparin II to inhibit thrombin activity, but not on cofactors VIII and IX. Therefore, the polysaccharide from H. floresia interfered on coagulation cascade.
Tipo: | Bioprospecção
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6402

10.4025/actascibiolsci.v33i3.6402

Editor: | Universidade Estadual De Maringá

Formato:

application/pdf

application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; v. 33, n. 3 (2011); 255-261

Acta Scientiarum. Biological Sciences; v. 33, n. 3 (2011); 255-261

Fonte:

DECLARATION OF ORIGINALITY AND CESSATION OF COPYRIGHTI Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. I also declare that once published in the Acta Scientiarum. Biological Sciences, a publication of the Universidade Estadual de Maringá, it will not be submitted by me or by any co-author to any other scientific communication mean. In my name and in the name of co-authors, I shall cede the copyright of the above mentioned article to the Universidade Estadual de Maringá and I declare that I know that the non-observance of this norm may make me liable for the penalties contemplated in the Law for the Protection of Authors’ Rights (Act 9609 of the 19th February 1998).

DECLARAÇÃO DE ORIGINALIDADE E CESSÃO DE DIREITOS AUTORAIS  Declaro que o presente artigo é original, não tendo sido submetido à publicação em qualquer outro periódico nacional ou internacional, quer seja em parte ou em sua totalidade. Declaro, ainda, que uma vez publicado na revista Acta Scientiarum. Biological Sciences, editada pela Universidade Estadual de Maringá, o mesmo jamais será submetido por mim ou por qualquer um dos demais co-autores a qualquer outro meio de divulgação científica. Através deste instrumento, em meu nome e em nome dos demais co-autores, porventura existentes, cedo os direitos autorais do referido artigo à Universidade Estadual de Maringá e declaro estar ciente de que a não observância deste compromisso submeterá o infrator a sanções e penas previstas na Lei de Proteção de Direitos Autorias (Nº9609, de 19/02/98).