Data/hora: 13/06/2024 11:03:29

Provedor de dados: | 3
País: | Brazil

Título:

Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas

Genetic diversity of 16S rRNA and mcrA genes from methanogenic archaeas

Autores: | Xavier, Keyla Vitoria Marques; Souza, Eden Silva e; Santos, Erick de Aquino; Diniz, Michely Correia.
Data: | 2020-04-03
Ano: | 2020
Palavras-chave: | Heterozygosity; Microbiomes; Methane; Anaerobic; Arlerquin; Databases heterozygosity; Microbiomes; Methane; Anaerobic; Arlerquin; Databases.
Resumo: | Methanogenic archaeas are found in aquatic and terrestrial environments and are fundamental in the conversion of organic matter into methane, a gas that has a potential use as renewable source of energy, which is also considered as one of the main agents of the greenhouse effect. The vast majority of microbial genomes can be identified by a conservative molecular marker, the 16S ribosomal gene. However, the mcrA gene have been using in studies of methanogenic archaea diversity as an alternative marker, highly conserved and present only in methanogens. This gene allows the expression of the enzyme Methyl-coenzyme M reductase, the main agent in converting by-products of anaerobic digestion into methane. In this context, we aimed to study the genetic diversity of mcrA and 16S rRNA genes sequences available in databases. The nucleotide sequences were selected from the NCBI. The heterozygosity and molecular diversity indexes were calculated using the Arlequin 3.5 software, with plots generated by package R v3.0. The diversity and heterozygosity indices for both genes may have been influenced by the number and size of the sequences. Descriptive analysis of genetic diversity generated by sequences deposited in databases allowed a detailed study of these molecules. It is known that the organisms in a population are genetically distinct, and that, despite having similarities in their gene composition, the differences are essential for their adaptation to different environments.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês

Identificador:

http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/49877

10.4025/actascibiolsci.v42i1.49877

Editor: | Universidade Estadual De Maringá
Relação: | http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/49877/751375149812
Formato: | application/pdf

Fonte:

Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e49877

Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e49877

1807-863X

1679-9283

Fonte:

Copyright (c) 2020 Acta Scientiarum. Biological Sciences

http://creativecommons.org/licenses/by/4.0