Data/hora: 10/06/2024 16:35:27

Provedor de dados: | 36
País: | Brazil
Título: | A feasible method to extract DNA from the cambium of high-canopy trees: from harvest to assessment
Autores: | MANGARAVITE,Érica; TERRA,Vanessa; HATTORI,Eric Koiti Okiyama; DAL’SASSO,Thaís Carolina da Silva; BHERING,Leonardo Lopes; OLIVEIRA,Luiz Orlando de.
Data: | 2020-12-01
Ano: | 2020
Palavras-chave: | Ascorbic acid; Dithiothreitol; DNA isolation.
Resumo: | ABSTRACT Many tropical trees have high canopies and their leaves are not accessible. Thus, the use of tissue from a more accessible organ (cambium) for DNA extraction may be an alternative for molecular studies. We adapted a feasible methodology for extracting genomic DNA from cambium tissue harvested in the field for the assessment with PCR. We tested three storage conditions (two buffers and a silica gel) and four periods of time after harvest. We used previously described protocols and tested them on three species that occur in Amazonian forests and other biomes: Anadenanthera peregrina var. peregrina, Cedrela fissilis, and Ceiba speciosa. Our protocol obtained suitable PCR-grade genomic DNA for DNA sequencing and microsatellite genotyping. We recommend the use of silica for long-term storage and the buffer with ascorbic acid for short-term storage.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400335
Editor: | Instituto Nacional de Pesquisas da Amazônia
Relação: | 10.1590/1809-4392202001571
Formato: | text/html
Fonte: | Acta Amazonica  v.50 n.4 2020
Fonte: | info:eu-repo/semantics/openAccess