Data/hora: 02/06/2024 06:41:10

Provedor de dados: | 42
País: | Brazil
Título: | Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus
Autores: | Alves,Gutemberg G.; Marinho-Carvalho,Monica M.; Atella,Georgia C.; Silva-Neto,Mario A.C.; Sola-Penna,Mauro.
Data: | 2007-03-01
Ano: | 2007
Palavras-chave: | Phosphofructokinase; Metabolism; Insect.
Resumo: | 6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, and citrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activity for each tissue, with regulatory patterns that are consistent with their physiological roles.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008
Editor: | Academia Brasileira de Ciências
Relação: | 10.1590/S0001-37652007000100008
Formato: | text/html
Fonte: | Anais da Academia Brasileira de Ciências  v.79 n.1 2007
Fonte: | info:eu-repo/semantics/openAccess