Data/hora: 10/06/2024 18:33:06

Provedor de dados: 	42	
País: 	Brazil	
Título: 	Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus	
Autores: 	Alves,Gutemberg G.; Marinho-Carvalho,Monica M.; Atella,Georgia C.; Silva-Neto,Mario A.C.; Sola-Penna,Mauro.	
Data: 	2007-03-01	
Ano: 	2007	
Palavras-chave: 	Phosphofructokinase; Metabolism; Insect.	
Resumo: 	6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, and citrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activity for each tissue, with regulatory patterns that are consistent with their physiological roles.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000100008	
Editor: 	Academia Brasileira de Ciências	
Relação: 	10.1590/S0001-37652007000100008	
Formato: 	text/html	
Fonte: 	Anais da Academia Brasileira de Ciências  v.79 n.1 2007	
Fonte: 	info:eu-repo/semantics/openAccess