Data/hora: 14/06/2024 05:20:43

Provedor de dados: | 45
País: | Brazil
Título: | Production of monoclonal antibodies against conserved components of infectious bronchitis virus
Autores: | Souza,C.M.; Rocha,F.R.T.; Martins,N.R.S.; Resende,J.S.; Jorge,M.A.; Rampinelli,A.P.
Data: | 2001-10-01
Ano: | 2001
Palavras-chave: | Chicken; Monoclonal antibody; Infectious bronchitis; Nucleoprotein; Spike glicoprotein S2.
Resumo: | Murine hybridomas producing IgG1 monoclonal antibodies (Mabs) against N and S2 proteins (53KDa and 82KDa, respectively) from avian infection bronchitis virus (IBV) strain M41 were generated by the fusion of a myeloma cell line (Sp2/0-Ag14) with spleen cells from Balb/c mice previously immunized with whole virus IBV M41. Post-fusion screening criterion was by ELISA and 36 positive hybrids were generated after fusions. Two hybrids specific to N (N3F10) and S2 (S12B2) proteins from M41 (serotype Massachusetts) were selected by western blotting. These Mabs recognized the Ark-99 (serotype Arkansas) and A5968 (serotype Connecticut) IBV strains in addition to M41. By ELISA, the Mab against the S2 (S12B2) recognized all reference and Brazilian strains (M41, SE-17, H52, 297, 283, PM-1, PM-2, PM-3, 351, 29-78 E 327) studied, while the Mab against N recognized only six (M41, SE-17, H52, 283, 327 e 297) strains. The Mab against S2 may become a useful tool for IBV detection on the routine diagnosis of infectious bronchitis, especially for helping the differential diagnosis of clinically and pathologically confusing diseases, while the Mab against N (N3F10) recognized a probably less conserved region among the strains and may be interesting to comparing IBV isolates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500002
Editor: | Universidade Federal de Minas Gerais, Escola de Veterinária
Relação: | 10.1590/S0102-09352001000500002
Formato: | text/html
Fonte: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia  v.53 n.5 2001
Fonte: | info:eu-repo/semantics/openAccess