Data/hora: 16/06/2024 01:23:22

Provedor de dados: | 47
País: | Argentina
Título: | Plant regeneration after long term callus culture in clones of Asparagus officinalis L.
Autores: | Pontaroli,A. C.; Camadro,E. L.
Data: | 2005-12-01
Ano: | 2005
Palavras-chave: | Callus; Shoot primordia induction; Rooting; Medium x genotype interactions.
Resumo: | Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l-1 2,4-D and 1 mg.l-1 kinetin. Shoot primordia (SP) induction from &gt;18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained with clone &amp;#8216;265' on MS medium with 5 mg.l-1 2iP, 1 mg.l-1 IAA, 3% sucrose and 0.9% agar. The highest percentage of root induction (100%) was achieved with clone &amp;#8216;265' on MS medium with 0.1 mg.l-1 kinetin, 0.1 mg.l-1 NAA, 1.32 mg.l-1 ancymidol, 7% glucose and 0.8% agar. Important medium x genotype interactions were detected, pointing to the need of adjusting this and other in vitro protocols for specific asparagus genotypes.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300009
Editor: | Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)
Formato: | text/html
Fonte: | Biocell  v.29 n.3 2005
Fonte: | info:eu-repo/semantics/openAccess