Data/hora: 13/06/2024 16:49:45

Provedor de dados: 	5	
País: 	France	
Título: 	Effects of extracellular products from the pathogenic Vibrio aestuarianus strain 01/32 on lethality and cellular immune responses of the oyster Crassostrea gigas	
Autores: 	Labreuche, Yannick; Soudant, Philippe; Goncalves, Madeleine; Lambert, Christophe; Nicolas, Jean-louis.	
Data: 	2006	
Ano: 	2006	
Palavras-chave: 	Vibrio aestuarianus; ECPs; Crassostrea gigas; ROS production; Adhesion; Phagocytosis; Flow cytometry; Bivalve immunity.	
Resumo: 	Vibrio aestuarianus strain 01/32 was previously shown to be pathogenic to Crassostrea gigas juveniles. To investigate virulence mechanisms of this pathogen, we studied the toxicity to oysters of its extracellular products (ECPs). ECPs displayed lethality to animals, with a LD50 value of 3.3 mu g/g body weight. To determine the oyster cellular immune responses induced by these ECPs, we further examined in vitro their effects on C. gigas hemocytes, using flow cytometric-based hemocyte assays. Treatment of hemolymph with ECPs caused a significant inhibition of hemocyte phagocytosis and adhesive capabilities. In contrast, the pathway of reactive oxygen species production was enhanced by higher ECP concentrations. Exposure of hemocytes to live bacteria induced no changes in hemocyte parameters. Together, these results suggest that V. aestuarianus strain 01/32 secretes one or more factors which may play an important role in the pathogenicity of this microorganism, and which display immunosuppressant activities on hemocyte functions. (c) 2005 Elsevier Ltd. All fights reserved.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/2006/publication-1035.pdf

DOI:10.1016/j.dci.2005.05.003	
Editor: 	Elsevier	
Relação: 	http://archimer.ifremer.fr/doc/00000/1035/	
Formato: 	application/pdf	
Fonte: 	Developmental &amp; Comparative Immunology (0145-305X) (Elsevier), 2006 , Vol. 30 , N. 4 , P. 367-379	
Fonte: 	2005 Elsevier Ltd All rights reserved