Data/hora: 10/06/2024 10:14:48

Provedor de dados: 	5	
País: 	France	
Título: 	The impact of mechanical compression on cortical microtubules in Arabidopsis: a quantitative pipeline	
Autores: 	Louveaux, Marion; Rochette, Sebastien; Beauzamy, Lena; Boudaoud, Arezki; Hamant, Olivier.	
Data: 	2016-10	
Ano: 	2016	
Palavras-chave: 	Mechanical stress; Indenter; Microtubules; Compression; Bundling; Arabidopsis thaliana; Technical advance.	
Resumo: 	Exogenous mechanical perturbations on living tissues are commonly used to investigate whether cell effectors can respond to mechanical cues. However, in most of these experiments, the applied mechanical stress and/or the biological response are described only qualitatively. We developed a quantitative pipeline based on microindentation and image analysis to investigate the impact of a controlled and prolonged compression on microtubule behaviour in the Arabidopsis shoot apical meristem, using microtubule fluorescent marker lines. We found that a compressive stress, in the order of magnitude of turgor pressure, induced apparent microtubule bundling. Importantly, that response could be reversed several hours after the release of compression. Next, we tested the contribution of microtubule severing to compression-induced bundling: microtubule bundling seemed less pronounced in the katanin mutant, in which microtubule severing is dramatically reduced. Conversely, some microtubule bundles could still be observed 16 hours after the release of compression in the spiral2 mutant, in which severing rate is instead increased. To quantify the impact of mechanical stress on anisotropy and orientation of microtubule arrays, we used the nematic tensor based FibrilTool ImageJ/Fiji plugin. To assess the degree of apparent bundling of the network, we developed several methods, some of which were borrowed from geostatistics. The final microtubule bundling response could notably be related to tissue growth velocity that was recorded by the indenter during compression. Because both input and output are quantified, this pipeline is an initial step towards correlating more precisely the cytoskeleton response to mechanical stress in living tissues.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00347/45829/45479.tiff

http://archimer.ifremer.fr/doc/00347/45829/45480.tif

http://archimer.ifremer.fr/doc/00347/45829/45481.tiff

http://archimer.ifremer.fr/doc/00347/45829/45482.tiff

http://archimer.ifremer.fr/doc/00347/45829/45483.pdf

http://archimer.ifremer.fr/doc/00347/45829/45484.pdf

http://archimer.ifremer.fr/doc/00347/45829/45863.pdf

DOI:10.1111/tpj.13290

http://archimer.ifremer.fr/doc/00347/45829/	
Editor: 	Wiley-blackwell	
Formato: 	application/pdf	
Fonte: 	Plant Journal (0960-7412) (Wiley-blackwell), 2016-10 , Vol. 88 , N. 2 , P. 328-342	
Fonte: 	2016 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley &amp; Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations ...

info:eu-repo/semantics/openAccess

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