Data/hora: 13/06/2024 10:31:20

Provedor de dados: | 5
País: | France
Título: | Déterminisme nutritionnel et génétique de la teneur en lipides musculaires chez la truite arc-en-ciel (Oncorhynchus mykiss) [Texte imprimé]  : étude par analyse de l'expression de gènes candidats, du protéome et du transcriptome du foie et du muscle
Autores: | Kolditz, Catherine-inès.
Data: | 2008-12-09
Ano: | 2008
Palavras-chave: | Proteomics; Transcriptomics; Selective breeding; Dietary energy; Rainbow trout; Protéome; Transcriptome; Sélection génétique; Apport énergétique alimentaire; Truite arc en ciel.
Resumo: | The objective of the study was to identify genes and proteins that are involved in the control of muscle fat deposition in rainbow trout. We analyzed the combined effects exerted by genetic selection and dietary treatment, which are the two main factors that can be used to manage body fat content. Two lines of rainbow trout, obtained after 3 generations of divergent selection for high or low muscle fat content, were fed diets containing either 10% or 23% lipids (% dry matter), for six months. We analyzed the activity and gene expression of key enzymes involved in energy utilization, and performed a more global approach through transcriptome (nylon microarray) and proteome (twodimensional electrophoresis) analysis. We analyzed the liver, which is the centre of intermediary metabolism and the main site of lipogenesis in fish, and the muscle, the target tissue of the selection provedure. The results confirmed the depressing effect exerted by a lipid rich diet on lipogenesis and  fatty acid desaturation, already described in larger size fish, and provided new insight about the effect exerted on the other metabolic pathways, in particular the proteolysis. These analyses pointed out metabolic differences existing between lines. They involved not only lipid metabolism, but also the other pathways of nutrient utilization. With regard to their muscle-fattening effect, the dietary treatment and the genetic selection appear to act through different molecular mechanisms. These analyses allowed the identification of two genes that are over-expressed in muscle upon both high dietary lipid supply and upward selection for muscle fat content, suggesting that these two genes could be relevant molecular markers of muscle fattening.
Tipo: | Text
Idioma: | Francês
Identificador: | http://archimer.ifremer.fr/doc/2008/these-6794.pdf
Editor: | Bordeaux 1
Relação: | http://archimer.ifremer.fr/doc/00000/6794/
Formato: | application/pdf