Data/hora: 10/06/2024 04:31:17

Provedor de dados: 	5	
País: 	France	
Título: 	A direct monoclonal antibody sandwich immunoassay for detection of Bonamia ostreae (Ascetospora) in hemolymph samples of the flat oyster Ostrea edulis (Mollusca : Bivalvia)	
Autores: 	Cochennec, Nathalie; Hervio, Dominique; Panatier, B; Boulo, Viviane; Mialhe, Eric; Rogier, H; Grizel, Henri; Paolucci, F.	
Data: 	1992-02	
Ano: 	1992	
Resumo: 	Currently recognition of molluscan diseases relies heavily upon light microscopic examination of stained histological sections of molluscan tissues Such histopathological methods are time consuming and relatively inefficient In the study of ongoing epizootics Alternatively, more effluent investigative tools are needed to accelerate disease recognition and quantification Such methods are needed to prevent control and eradicate molluscan diseases This paper reports the development of a direct sandwich enzyme-lmmunosorbent assay (ELISA) for the ascetosporan intrahemocytic parasite Bonamiao ostreae of the flat oyster Ostrea edulis. Bonamiasis is a commercially important epizootic disease of oysters A Bonamia-specific monoclonal antibody is employed in the assay The assay is performed In antibody-coated microplates employing standardized reagent and 50 µl oyster hemolymph samples This procedure was developed into a commercial product, available as a kit Based upon clinical studies, there was a 90 O/o agreement In test results when ELISAs were compared to the reference histopathologic light microscopic examination for the parasites	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/1992/publication-2765.pdf	
Editor: 	Inter-research	
Relação: 	http://archimer.ifremer.fr/doc/00000/2765/	
Formato: 	application/pdf	
Fonte: 	Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1992-02 , Vol. 12 , N. 2 , P. 129-134	
Fonte: 	Inter-Research