Data/hora: 03/06/2024 03:25:48

Provedor de dados: | 5
País: | France
Título: | Recherche et identification de bactéries pathogènes au sein d'une écloserie nurserie d'huître creuse, Crassostrea gigas, en relation avec la mortalité estivale du naissain
Autores: | Waechter, Magali.
Data: | 2001-06-18
Ano: | 2001
Palavras-chave: | Mortalité estivale; Naissain; Crassostrea gigas; Ecloserie; Nursery; France; Bactériologie; Bactérie; Vibrio; Vibrio splendidus biovar II; Summer mortality; Oyster spat; Crassostrea gigas; Hatchery; Nursery; France; Bacteriology; Bacteria; Vibrio; Vibrio splendidus biovar II.
Resumo: | Summer mortality has been observed on oyster spat Crassostrea gigas since 1991 on french coasts. In our study, we deseribed symptoms observed during the mortality, especially adductor muscle weakness and we isolated strains on Zobell medium and TCBS from moribund spat. Strains isolated during a high mortality (60 %) were selected by experimental pathology on spat. Inoculation by bath led us to select a pathogenic strain (TNEMF6) for oyster spat. Phenotypic identification, phylogenetic study on 16S rDNA and quantitative DNA/DNA hybridisation showed that this strain belongs to Vibrio splendidus biovar II. Phenotyping and ribotyping with strains isolated during the study showed that only TNNIII7 and TNMB5 are similar to the pathogenic strain TNEMF6. Quantitative DNA/DNA hybridisation showed that only TNNIII7 belongs to the same specie than TNEMF6. Difficulty to isolate these strains of Vibrio splendidus II suggested that they may be located out of the tissues. A pathogenic effect with proteolytie exotoxins is suspected related to the weakness of the adductor muscle observed on moribund oyster spat. Then we project to search for detection probes specific to Vibrio splendidus biovar II and pathogenic strains of this specie
Tipo: | Text
Idioma: | Francês

Identificador:

http://archimer.ifremer.fr/doc/00211/32226/30682.pdf

http://archimer.ifremer.fr/doc/00211/32226/

Editor: | Université de La Rochelle
Formato: | application/pdf

Fonte:

2001 Université de La Rochelle

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