Data/hora: 13/06/2024 13:46:43

Provedor de dados: | 5
País: | France
Título: | Survey for neoplasia in Macoma balthica from the Gulf of Gdansk by flow cytometry
Autores: | Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek.
Data: | 2005-08
Ano: | 2005
Palavras-chave: | Prevalence; Macoma balthica; Flow cytometry; Gill neoplasia.
Resumo: | Using flow cytometry, 234 Macoma balthica were examined during a survey to determine frequency of neoplasia in the Gulf of Gdansk (Poland). Clams were collected in 4 locations and DNA content in gill tissue cells was determined by flow cytometry using propidium iodide staining. Cell permeabilization was induced by osmotic shock. Prevalence of neoplasia ranged from 9.6 to 26.7 % depending on location. DNA content in aneuploid cells was higher than in normal dividing cells. The fluorescence value for aneuploid cells corresponded to tetraploid/pentaploid cells. Three stages of neoplasia were defined, based on the percentage of aneuploid cells determined by flow cytometry. Histopathological and cytogenetic analyses were also carried out on the same clams for comparative study. Proportions of normal and affected clams detected using flow cytometry were similar to those identified using both methods. In the present study, no clear relationship was demonstrated between prevalence of neoplasia and pollutant detection in the different sampling sites.
Tipo: | Text
Idioma: | Inglês

Identificador:

http://archimer.ifremer.fr/doc/2005/publication-2919.pdf

DOI:10.3354/dao066041

Editor: | Inter-Research
Relação: | http://archimer.ifremer.fr/doc/00000/2919/
Formato: | application/pdf
Fonte: | Diseases of aquatic organisms (0177-5103) (Inter-Research), 2005-08 , Vol. 66 , N. 1 , P. 41-46
Fonte: | 2005 Inter-Research