Data/hora: 03/06/2024 03:01:29

Provedor de dados: 	5	
País: 	France	
Título: 	Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis	
Autores: 	Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C.	
Data: 	2003-12	
Ano: 	2003	
Palavras-chave: 	Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity.	
Resumo: 	Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00000/10761/7590.pdf

DOI:10.3354/dao057109	
Editor: 	Inter-research	
Relação: 	http://archimer.ifremer.fr/doc/00000/10761/	
Formato: 	application/pdf	
Fonte: 	Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2003-12 , Vol. 57 , N. 1-2 , P. 109-116