Data/hora: 05/06/2024 02:48:22

Provedor de dados: 	5	
País: 	France	
Título: 	Determination of reduced sulfur compounds by high-performance liquid chromatography in hydrothermal seawater and body fluids from Riftia pachyptila	
Autores: 	Gru, Cecile; Sarradin, Pierre-marie; Legoff, Hélène; Narcon, Sandrine; Caprais, Jean-claude; Lallier, Francois.	
Data: 	1998-06	
Ano: 	1998	
Palavras-chave: 	Sulfide; Sulfite; Thiosulfate; Cysteine; Glutathione; Hydrothermal seawater; Riftia pachyptila; Biological fluids.	
Resumo: 	This paper describes a method for the determination of reduced sulfur compounds in hydrothermal seawater and body fluids from the hydrothermal tube worm Riftia pachyptila. Sulfur is a hey component of the hydrothermal ecosystem based on chemosynthesis, Sulfur compounds were derivatized at pH 8 (4.3 for sulfide in biological fluid) with a fluorescent reagent, monobromobimane, and separated by reverse-phase HPLC. The eluted compounds were detected by spectrofluorimetry. This method allowed: the quantitative analysis of sulfide, sulfite, thiosulfate, cysteine and glutathione in seawater, vascular blood and coelomic fluids from R, pachyptila, The detection limits were in the 0.1 mu mol l(-1) range with a precision lower than 10%, The method has been applied to hydrothermal seawater. The organisms are distributed along a gradient of sulfide (produced by the vent) and thiosulfate. Analysis of biological fluid was performed with a new sample treatment allowing the analysis of total sulfide (free and bound to haemoglobin) with results comparable to published methods.	
Tipo: 	Text	
Idioma: 	Inglês	
Identificador: 	http://archimer.ifremer.fr/doc/00186/29763/28203.pdf

DOI:10.1039/a800032h	
Editor: 	Royal Soc Chemistry	
Relação: 	http://archimer.ifremer.fr/doc/00186/29763/	
Formato: 	application/pdf	
Fonte: 	Analyst (0003-2654) (Royal Soc Chemistry), 1998-06 , Vol. 123 , N. 6 , P. 1289-1293