Data/hora: 13/06/2024 12:50:09

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation	
Autores: 	Guelfi,Marieli; Maioli,Marcos Antonio; Tavares,Marco Aurélio; Mingatto,Fábio Erminio.	
Data: 	2015-12-01	
Ano: 	2015	
Palavras-chave: 	Fipronil; Hepatotoxicity; Bioenergetics; Biotransformation; Calcium homeostasis.	
Resumo: 	ABSTRACT The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in a dose-dependent manner and dissipated the mitochondrial membrane potential that was accompanied by a reduction in ATP concentration and a disruption of intracellular Ca2+ homeostasis. The cell viability was affected by fipronil with higher potency in hepatocytes that were isolated from the normal rats, which indicated that the metabolism of this insecticide increased its toxicological potential. The results of this study indicated that the toxicity of fipronil to the hepatocytes was related to the inhibition of mitochondrial activity, which led to decreased ATP synthesis and a consequent alteration in intracellular Ca2+ homeostasis and ultimately resulted in cell death.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600843	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132015060298	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.58 n.6 2015	
Fonte: 	info:eu-repo/semantics/openAccess