Data/hora: 02/06/2024 07:16:05

Provedor de dados: | 52
País: | Brazil
Título: | Biochemical and morphological responses to abiotc elicitor chitin in suspension-cultured sugarcane cells
Autores: | Gallão,Maria Izabel; Cortelazzo,Ângelo Luiz; Fevereiro,Manuel Pedro Salema; Brito,Edy Sousa de.
Data: | 2010-04-01
Ano: | 2010
Palavras-chave: | Chitin; Elicitation; Peroxidase; PAL; Sugarcane; Tissue culture.
Resumo: | Cells of Saccharum officinarum submitted to hydrolyzated chitin for 1 to 8h produced phenolic compounds. These alterations were observed through cytochemical methods using Toluidine Blue and Phloroglucinol/HCl. After 4 h, besides cell wall change, there was a change in nuclear pattern of chitin treated cells. There was a 96% increase in nuclear area in 6 h chitin treated material, as observed by Feulgen reaction. The treated cells showed chromatin compacted regions and a degeneration process of nucleoli. In the outer areas of cell wall, there was a polysaccharide desagregation, confirming results obtained for different plants with the use of other elicitors. Peroxidase activity was maximal after 4 h and decreased progressively. PAL activity started to increase at 4 h of incubation. These results showed that chitin hydrolyzate stimulated a defense response in sugarcane cells.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200002
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000200002
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess