Data/hora: 13/06/2024 11:48:34

Provedor de dados: | 52
País: | Brazil
Título: | Detection of Clostridium sp. and its Relation to Different Ages and Gastrointestinal Segments as Measured by Molecular Analysis of 16S rRNA Genes
Autores: | Mirhosseini,Seyed Ziaeddin; Seidavi,Alireza; Shivazad,Mahmoud; Chamani,Mohammad; Sadeghi,Ali Asghar; Pourseify,Reza.
Data: | 2010-02-01
Ano: | 2010
Palavras-chave: | Clostridium spp.; Broiler; Gastrointestinal; Age; 16S rRNA genes.
Resumo: | The objective of this study was to establish a specific, sensitive and rapid PCR approach for the detection of Clostridium sp. at the genus level. Clostridium sp. in the duodenum, jejunum, ileum and cecum of broiler chickens were analyzed by 16S rRNA genes. The PCR detected the presence of Clostridium spp. in naturally contaminated intestinal samples. For the total gastrointestinal segments, 53.125, 65.625 and 59.375% samples were positive for naturally occurring Clostridium spp. at the ages 4, 14 and 30d, respectively. Analysis of the microbial contents indicated that Clostridium sp. was not consistently detected in all intestinal segments. These results can put in evidence the hypothesis that Clostridium spp. may be interfering in health and performance of chickens.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100009
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000100009
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.1 2010
Fonte: | info:eu-repo/semantics/openAccess