Data/hora: 05/06/2024 18:30:16

Provedor de dados: | 52
País: | Brazil
Título: | A study of ion-exchange chromatography in an expanded bed for bovine albumin recovery
Autores: | Severo Jr.,João Batista; Souza,Roberto Rodrigues de; Santana,José Carlos Curvelo; Tambourgi,Elias Basile.
Data: | 2009-04-01
Ano: | 2009
Palavras-chave: | BSA; Protein adsorption; Breakthrough curves; Amberlite IRA 410 ion-exchange resin; Distribution residence time study; Richardson-Zack coefficient.
Resumo: | In the present work, the effect of bed expansion on BSA adsorption on Amberlite IRA 410 ion-exchange resin was studied. The hydrodynamic behavior of an expanded bed adsorption column on effects of the biomolecules and salt addition and temperature were studied to optimize the conditions for BSA recovery on ion-exchange resin. Residence time distribution showed that HEPT, axial dispersion and the Pecletl number increased with temperature and bed height, bed voidage and linear velocity. The binding capacity of the resin increased with bed height. The Amberlite IRA 410 ion-exchange showed an affinity for BSA with a recovery yield of 78.36 % of total protein.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200021
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132009000200021
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.52 n.2 2009
Fonte: | info:eu-repo/semantics/openAccess