Data/hora: 10/06/2024 17:17:17

Provedor de dados: | 52
País: | Brazil
Título: | Targeting of Virulence Factors and Plasmid Profiling of Klebsiella pneumoniae Causing Urinary Tract Infection in Sylhet City of Bangladesh
Autores: | Foysal,Md Javed; Majlish,Al-Nahian Khan; Islam,Kamrul; Alam,Md Jahangir; Ali,Md Hazrat; Momtaz,Farhana.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | K. pneumoniae; Urinary tract infection; Virulence factors; Plasmid profiling; Correlation study.
Resumo: | ABSTRACT Studies were conducted to characterize Klebsiella pneumoniae isolates from urinary tract infection (UTI) patients in Sylhet city of Bangladesh. At the same time, all isolates were screened for some common virulence genes and four significant isolates were searched for plasmid number and sizes by mini alkaline-lysis method. Among five tested isolates from female UTI patients, gyrase subunit B2 (gyrb2) amplified in all isolates, lipase and nuclease detected in three isolates and serine protease amplifies in two isolates and gave the expected band of 1130 bp, 517 bp, 1055 bp and 211 bp respectively. Two of four isolates showed 9.82 kb plasmid band on agarose gel. Isolates bearing 9.82 kb plasmid were found to be resistant to multiple commercial antibiotics. At the same time all isolates were screened for in-vitro plate assay for proteolytic, lypolytic and hemolytic activity. Isolates with positive plasmid and more than one virulent gene with gyrB2 showed positive result in in-vitro culture plate with clear zone of proteolysis, hemolysis or lipolysis. This study will be helpful for further study in finding correlation or pattern of virulence properties for K. pneumoniae associated UTI in Bangladesh.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100423
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/1678-4324-2018160643
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.61 2018
Fonte: | info:eu-repo/semantics/openAccess