Data/hora: 01/06/2024 01:33:40

Provedor de dados: | 52
País: | Brazil
Título: | Adsorption of amyloglucosidase from Aspergillus niger NRRL 3122 using ion exchange resin
Autores: | Manera,Ana Paula; Kamimura,Eliana Setsuko; Brites,Luciana Machado; Kalil,Susana Juliano.
Data: | 2008-10-01
Ano: | 2008
Palavras-chave: | Amyloglucosidase; Adsorption isotherm; Kinetics adsorption; Ion exchange resin; Purification.
Resumo: | Amyloglucosidase enzyme was produced by Aspergillus niger NRRL 3122 from solid-state fermentation, using deffated rice bran as substrate. The effects of process parameters (pH, temperature) in the equilibrium partition coefficient for the system amyloglucosidase - resin DEAE-cellulose were investigated, aiming at obtaining the optimum conditions for a subsequent purification process. The highest partition coefficients were obtained using 0.025M Tris-HCl buffer, pH 8.0 and 25ºC. The conditions that supplied the highest partition coefficient were specified, the isotherm that better described the amyloglucosidase process of adsorption obtained. It was observed that the adsorption could be well described by Langmuir equation and the values of Qm and Kd estimated at 133.0 U mL-1 and 15.4 U mL-1, respectively. From the adjustment of the kinetic curves using the fourth-order Runge-Kutta algorithm, the adsorption (k1) and desorption (k2) constants were obtained through optimization by the least square procedure, and the values calculated were 2.4x10-3 mL U-1 min-1 for k1 and 0.037 min-1 for k2 .
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500019
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132008000500019
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.51 n.5 2008
Fonte: | info:eu-repo/semantics/openAccess