Data/hora: 04/06/2024 04:19:44

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Chemotaxonomic Fingerprinting of Chilean Lichens Through Maldi and Electrospray Ionization Mass Spectrometry	
Autores: 	Santos,Leonardo Silva; Soriano,Maria del Pilar Camarantin; Mirabal-Gallardo,Yaneris; Carrasco-Sanchez,Veronica; Nachtigall,Fabiane Manke; Pereira,Iris; Pereira,Eugenia.	
Data: 	2015-04-01	
Ano: 	2015	
Palavras-chave: 	Chilean lichens; Chemotaxonomic; Fingerprint; MALDI-TOF-MS; UPLC-ESI(-)-MS.	
Resumo: 	The aim of this work was to study a fast, new, sensitive, and simple method for the chemotaxonomic classification of Chilean lichens (Teloschistes chrysophthalmus, Ramalina farinacea, Usnea pusilla, Ramalina chilensis and Stereocaulon ramulosum) using MALDI-TOF-MS and UPLC-ESI(-)-MS data. Lichens soluble proteins fingerprints were acquired by MALDI-TOF-MS and they were analyzed by chemometric (PCA). Lichens organic extracts fingerprints were obtained by UPLC-ESI(-)-MS. MALDI-TOF-MS associated with chemometric analysis was used to detect new m/z patterns of soluble proteins that were compared with Protein Data Bank of UnitPro. These data also permitted the satisfactory distinction among the families and species. UPLC-ESI(-)-MS fingerprints analyses of the organic extracts showed the presence of five major lichen compounds (atranorin, parietin, teloschistin, ramalinolic and usnic acids). In contrast to other techniques, MALDI-TOF-MS associated with chemometric analysis and UPLC-ESI(-)-MS provided a new, fast and sensitive method for chemotaxonomic characterization of lichens.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200244	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-8913201400185	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.58 n.2 2015	
Fonte: 	info:eu-repo/semantics/openAccess