Data/hora: 01/06/2024 00:26:44

Provedor de dados: | 52
País: | Brazil
Título: | An inexpensive alternative equipment for the plant material embedding in the paraffin under the vacuum
Autores: | Leitão,Carlos André Espolador; Cortelazzo,Angelo Luiz.
Data: | 2008-10-01
Ano: | 2008
Palavras-chave: | Embedding; Paraffin method; Plant anatomy; Technique in microscopy.
Resumo: | The present work describes an equipment constructed using inexpensive material for embedding the plant material in the paraffin under the vacuum, using an oven and a vacuum pump. The equipment was tested using the samples of Rodriguezia venusta (Orchidaceae) buds embedded in paraffin, where half of the samples were submitted to the vacuum by the equipment during the embedding. The material was sectioned with a rotary microtome, obtaining full series of quality sections. The control was hard to section with the microtome, obtaining damaged sections due the air bubbles, making the ribbon formation difficult. These results proved the effectiveness of the equipment, making it a practical, inexpensive and more portable solution for newly established laboratories.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500018
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132008000500018
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.51 n.5 2008
Fonte: | info:eu-repo/semantics/openAccess