Data/hora: 02/06/2024 09:47:39

Provedor de dados: | 52
País: | Brazil
Título: | Methodological variations in the isolation of genomic DNA from Streptococcus bacteria
Autores: | Moreira,Mônica; Noschang,Juliana; Neiva,Ivana Froede; Carvalho,Yanê; Higuti,llma Hiroko; Vicente,Vânia Aparecida.
Data: | 2010-08-01
Ano: | 2010
Palavras-chave: | Streptococcus; DNA isolation; CTAB; Ultrasound.
Resumo: | In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000400013
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess