Data/hora: 10/06/2024 10:05:10

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Search for alkaloids on callus culture of Passiflora alata	
Autores: 	Machado,Michelli Wesz; Stern Neto,Carlos; Salgado,Juliana; Zaffari,Gilmar; Barison,Andersson; Campos,Francinete Ramos; Corilo,Yuri Eberlim de; Eberlin,Marcos Nogueira; Biavatti,Maique Weber.	
Data: 	2010-08-01	
Ano: 	2010	
Palavras-chave: 	Passiflora alata; Β-carboline alkaloids; Pyrazoles; Callus culture.	
Resumo: 	Preliminary work on Passiflora alata leaves failed to detect harmane alkaloids using LC. The aim of this work was to investigate the production of harmane alkaloids through the cell culture of P. alata, inducing its precursor (L-tryptophan). The leaf explants presented satisfactory results after disinfection, and the callus formation was initiated in MS media with adequate quantities of phytohormones. Sixty days after inoculation, calli were inoculated in the optimized semi-solid MS media, with and without the addition of L-tryptophan (50, 100, 200 mg/L) and kept in standard conditions for 90 days. Calli were collected on days 6, 16, 26, 36, and 90, followed by acid-base extraction, and analysed by LC. The results showed an absence of harmane, harmin, harmol, harmalol, and harmaline. With L-tryptophan feeding, two peaks were detected, collected and analysed through positive mode electrospray [ESI(+)-MS] and sequential analysis in tandem ESI(+)-MS/MS. The spectra obtained were very similar, with a repetition of the more intense ions, and consecutive loss of 68 Da units, attributed to the heterocycle pyrazole. It appeared that this transformation was not related to any enzymatic pathway previously described for the plant from L-tryptophan, and the biosynthesis of β-carboline alkaloids in callus culture of P. alata were not observed in this work.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400020	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132010000400020	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.53 n.4 2010	
Fonte: 	info:eu-repo/semantics/openAccess