Data/hora: 10/06/2024 15:57:41

Provedor de dados: 	55	
País: 	Brazil	
Título: 	Genetic diversity of Plasmodium vivax and Plasmodium falciparum in Kohat District, Pakistan	
Autores: 	Khatoon,Lubna; Khan,Inam Ullah; Shah,Shahid Ali; Jan,Muhammad Ishtiaq; Ullah,Farhat; Malik,Salman Akbar.	
Data: 	2012-04-01	
Ano: 	2012	
Palavras-chave: 	Plasmodium vivax; Plasmodium falciparum; Genetic variation; Pakistan.	
Resumo: 	Malaria is one of the serious diseases threatening human health in Pakistan and contributes to a large proportion of the total malaria deaths in South Asia. However, little is known about the nature and extent of genetic diversity of the malarial parasites circulating in Pakistan. This study was designed to assess the infection status of Plasmodium and the genetic diversity of Plasmodium vivax and Plasmodium falciparum by analyzing msp-3α, msp-3β and msp-1, msp-2 genes respectively using allele specific nested PCR and RFLP assays. For this purpose, 130 field isolates were collected from the individuals who exhibited clinical symptoms associated with malaria in the Kohat region of Khyber Pakhtoonkhwa (KPK), Pakistan. Among 130 blood samples collected, P. vivax was detected in 105/130 (80.8%) and P. falciparum in 21/130 (16.2%). Mixed infections with both parasites were detected in 4/130 (3%) of the isolates. A large number of distinguishable alleles were found for msp genetic markers: 10 alleles for msp-3α and seven for msp-3β with one mixed infection in case of msp-3β. The genotyping of P. falciparum showed that K1+MAD20 mixed genotype was dominant in msp-1 and FC27 in msp-2. The results collectively suggest that P. vivax and P. falciparum populations in this region are highly polymorphic and mixed infections are prevalent.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200014	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1590/S1413-86702012000200014	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.16 n.2 2012	
Fonte: 	info:eu-repo/semantics/openAccess