Data/hora: 05/06/2024 00:52:10

Provedor de dados: 	56	
País: 	Brazil	
Título: 	Effect of actinomycin D on simian rotavirus (SA11) replication in cell culture	
Autores: 	Stefanelli,C.C.; Castilho,J.G.; Botelho,M.V.J.; Linhares,R.E.C.; Nozawa,C.M.	
Data: 	2002-04-01	
Ano: 	2002	
Palavras-chave: 	Rotavirus; Actinomycin D; Replication; Cell culture.	
Resumo: 	Rotaviruses are the major cause of viral diarrhea in humans and animals. Actinomycin D (Act D) is an antibiotic that intercalates DNA and therefore inhibits DNA-dependent transcription. The current study was carried out to assess the influence of Act D on the replication of simian rotavirus (SA11) in cell culture. Virus-infected MA-104 cell cultures were studied in the presence of Act D at concentrations of 1.25 and 2.5 µg/ml. Treatment of rotavirus-infected cells with 2.5 µg/ml Act D 48 h post-infection reduced the cytoplasmic metachromasia after staining with acridine orange by 25%. Viral RNA labeled with ³H-uridine in the presence of the drug was separated by polyacrylamide gel electrophoresis. Viral RNA replication was not affected by Act D, but increased ³H-uridine uptake was demonstrable by infected cells in the presence of the drug. This possibly was due to the inhibition of cellular RNA synthesis by Act D, which thus enhances incorporation of the radionuclide into the viral RNA. Act D reduced the number of infected cells presenting virus-specific fluorescence 48 h post-infection by more than 50%. These data suggest that Act D may have complexed with viral RNA and prevented newly synthesized mRNA from being translated, but may not have prevented early replication.	
Tipo: 	Info:eu-repo/semantics/other	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400006	
Editor: 	Associação Brasileira de Divulgação Científica	
Relação: 	10.1590/S0100-879X2002000400006	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Medical and Biological Research  v.35 n.4 2002	
Fonte: 	info:eu-repo/semantics/openAccess