Data/hora: 12/06/2024 13:45:20

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Xylanase production by Aspergillus awamori under solid state fermentation conditions on tomato pomace	
Autores: 	Umsza-Guez,Marcelo A.; Díaz,Ana B.; Ory,Ignacio de; Blandino,Ana; Gomes,Eleni; Caro,Ildefonso.	
Data: 	2011-12-01	
Ano: 	2011	
Palavras-chave: 	Solid state fermentation (SSF); Tomato pomace; Hydrolytic enzymes; Plate-type bioreactor; Xylanase production.	
Resumo: 	In this work, tomato pomace, a waste abundantly available in the Mediterranean and other temperate climates agro-food industries, has been used as raw material for the production of some hydrolytic enzymes, including xylanase, exo-polygalacturonase (exo-PG), cellulase (CMCase) and α-amylase. The principal step of the process is the solid state fermentation (SSF) of this residue by Aspergillus awamori. In several laboratory experiments, maximum xylanase and exo-PG activities were measured during the first days of culture, reaching values around 100 and 80 IU/gds (international units of enzyme activity per gram of dried solid), respectively. For CMCase and α-amylase production remained almost constant along fermentation, with average values of 19 and 21.5 IU/gds, respectively. Experiments carried out in a plate-type bioreactor at lab scale showed a clear positive effect of aeration on xylanase and CMCase, while the opposite was observed for exo-PG and α-amylase. In general, xylanase was the enzyme produced in higher levels, thus the optimum conditions for the determination of the enzyme activity was characterized. The xylanase activity shows an optimum pH of 5 and an optimum temperature of 50 ºC. The enzyme is activated by Mg2+, but strongly inhibited by Hg2+ and Cu2+. The enzymatic activity remains quite high if the extract is preserved in a range of pH from 3 to 10 and a temperature between 30 ºC to 40 ºC.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400046	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822011000400046	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.42 n.4 2011	
Fonte: 	info:eu-repo/semantics/openAccess