Data/hora: 10/06/2024 11:57:46

Provedor de dados: | 58
País: | Brazil
Título: | Cgl-SLT2 is required for appressorium formation, sporulation and pathogenicity in Colletotrichum gloeosporioide
Autores: | Yong,H.Y.; Bakar,F.D.A.; Illias,R.M.; Mahadi,N.M.; Murad,A.M.A.
Data: | 2013-12-01
Ano: | 2013
Palavras-chave: | Colletotrichum gloeosporioides; MAP kinase; Pathogenicity; Appressorium.
Resumo: | The mitogen-activated protein (MAP) kinase pathways has been implicated in the pathogenicity of various pathogenic fungi and plays important roles in regulating pathogenicity-related morphogenesis. This work describes the isolation and characterization of MAP kinase gene, Cgl-SLT2, from Colletotrichum gloeosporioides. A DNA sequence, including 1,633 bp of Cgl-SLT2 open-reading frame and its promoter and terminator regions, was isolated via DNA walking and cloned. To analyze gene function, a gene disruption cassette containing hygromycin-resistant gene was constructed, and Cgl-SLT2 was inactivated via gene deletion. Analysis on Cgl-slt2 mutant revealed a defect in vegetative growth and sporulation as compared to the wild-type strain. When grown under nutrient-limiting conditions, hyperbranched hyphal morphology was observed in the mutant. Conidia induction for germination on rubber wax-coated hard surfaces revealed no differences in the percentage of conidial germination between the wild-type and Cgl-slt2 mutant. However, the percentage of appressorium formation in the mutant was greatly reduced. Bipolar germination in the mutant was higher than in the wild-type at 8-h post-induction. A pathogenicity assay revealed that the mutant was unable to infect either wounded or unwounded mangoes. These results suggest that the Cgl-SLT2 MAP kinase is required for C. gloeosporioides conidiation, polarized growth, appressorium formation and pathogenicity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400031
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822013000400031
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.44 n.4 2013
Fonte: | info:eu-repo/semantics/openAccess