Data/hora: 01/06/2024 01:13:05

Provedor de dados: | 58
País: | Brazil
Título: | 16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains
Autores: | Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Data: | 2007-03-01
Ano: | 2007
Palavras-chave: | Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Resumo: | Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of variation than that found in 16S gene sequences, with similarity values ranging from 0.79-1.00. The dendogram based on similarity data revealed 5 main groups. This spacer sequence contains two genes for tRNA (tRNAala and tRNAile). The sequence analysis of the tRNA content showed a conserved region with a few differences in the nucleotide composition.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822007000100033
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.38 n.1 2007
Fonte: | info:eu-repo/semantics/openAccess