Data/hora: 10/06/2024 12:13:23

Provedor de dados: | 58
País: | Brazil
Título: | Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis
Autores: | Salles,Joana Falcão; Gitahy,Patrícia de Medeiros; Skøt,Leif; Baldani,José Ivo.
Data: | 2000-09-01
Ano: | 2000
Palavras-chave: | Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Endophytic bacteria; Diazotrophic; Cry gene.
Resumo: | The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G. diazotrophicus the Western-blot technique was used. In both cases, a specific antibody raised against the B. thuringiensis toxin was applied. The delta-endotoxin production showed by the G. diazotrophicus recombinant strain was dependent on the nitrogen fixing conditions since the cry3A gene was fused to a nif promoter. In the case of H. seropedicae the delta-endotoxin expression was not affected by the promoter (rhi) used. These results suggest that endophytic diazotrophic bacteria can be used as vectors to express entomopathogenic genes envisaging control of sugarcane pests.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300001
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822000000300001
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.31 n.3 2000
Fonte: | info:eu-repo/semantics/openAccess