Data/hora: 10/06/2024 12:06:29

Provedor de dados: | 58
País: | Brazil
Título: | Cord formation and colony morphology for the presumptive identification of Mycobacterium tuberculosis complex
Autores: | Monteiro,Paulo Henrique Tasso; Martins,Maria Conceição; Ueki,Suely Yoko Mizuka; Giampaglia,Carmen Maria Saraiva; Telles,Maria Alice da Silva.
Data: | 2003-06-01
Ano: | 2003
Palavras-chave: | Mycobacterium tuberculosis; Identification; Cord formation; Culture.
Resumo: | The identification of Mycobacterium tuberculosis complex (MT), using non-molecular methods, is time-consuming. The objective of this study was to evaluate a screening test for the presumptive identification of MT, which could potentially decrease laboratory turn-around time for reporting preliminary results. From January 1998 to December 1999, 3056 cultures were analysed at the Mycobacterial Laboratory, Instituto Adolfo Lutz, São Paulo, Brasil. The screening test consisted of observation of colony morphology on Löwenstein Jensen medium and evaluation of cord formation on smear microscopy from those positive cultures. After the screening test, the cultures identified as non-tuberculous mycobacteria were identified to species by conventional methods (growth on culture and biochemical tests). Those identified as MT were submitted to drug susceptibility tests. The presumptive identification of MT using the proposed screening test, when compared with conventional tests, presented 98.9, 86.9, 97.8 and 93.0% of sensitivity, specificity, positive and negative predictive values, respectively. The conclusion is that it is possible to make a presumptive identification of MT using visual analysis of colony morphology and cord formation on microscopy examination. This method could be used to report the presumptive identification of MT and to guide laboratory decisions regarding susceptibility and identification tests with little cost and in a very practical way.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200016
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822003000200016
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.34 n.2 2003
Fonte: | info:eu-repo/semantics/openAccess