Data/hora: 13/06/2024 02:37:45

Provedor de dados: | 58
País: | Brazil
Título: | Production and properties of alpha-amylase from thermophilic Bacillus sp.
Autores: | Cordeiro,Carlos Alberto Martins; Martins,Meire Lelis Leal; Luciano,Angélica Bárbara.
Data: | 2002-01-01
Ano: | 2002
Palavras-chave: | Alpha-amylase; Thermophilic bacterium; Bacillus sp.
Resumo: | alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) production by thermophilic Bacillus sp strain SMIA-2 cultivated in liquid media containing soluble starch reached a maximum at 48h, with levels of 57U/mL. Studies on the a-amylase characterization revealed that the optimum temperature for activity was 70ºC. The enzyme was stable for 2h at 50ºC, while at 60ºC, 70ºC and 90ºC, 4%, 13% and 38% of the original activities were lost, respectively. The optimum pH of the enzyme was 7.5. After incubation of crude enzyme solution for 24h at pH 7.5, a decrease of about 5% of its original activity was observed. The enzyme was strongly inhibited by Co2+, Cu2+ and Ba2+, but less affected by Ca2+, Mg2+, Ni2+, Sr2+ and Mn2+. The enzyme in 1M and 5M NaCl solutions the enzyme retained 70% and 47% of the original activity after 24h of incubation at 4ºC, respectively.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100012
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822002000100012
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.33 n.1 2002
Fonte: | info:eu-repo/semantics/openAccess