Data/hora: 13/06/2024 05:22:41

Provedor de dados: | 58
País: | Brazil
Título: | Phenotypic, genetic and symbiotic characterization of Erythrina velutina rhizobia from Caatinga dry forest
Autores: | Rodrigues,Dalila Ribeiro; Silva,Aleksandro Ferreira da; Cavalcanti,Maria Idaline Pessoa; Escobar,Indra Elena Costa; Fraiz,Ana Carla Resende; Ribeiro,Paula Rose de Almeida; Ferreira Neto,Reginaldo Alves; Freitas,Ana Dolores Santiago de; Fernandes-Júnior,Paulo Ivan.
Data: | 2018-09-01
Ano: | 2018
Palavras-chave: | Biological nitrogen fixation; "Mulungu"; Diversity; Inoculant; 16S rRNA.
Resumo: | Abstract Erythrina velutina ("mulungu") is a legume tree from Caatinga that associates with rhizobia but the diversity and symbiotic ability of "mulungu" rhizobia are poorly understood. The aim of this study was to characterize "mulungu" rhizobia from Caatinga. Bacteria were obteined from Serra Talhada and Caruaru in Caatinga under natural regeneration. The bacteria were evaluated to the amplification of nifH and nodC and to metabolic characteristics. Ten selected bacteria identified by 16S rRNA sequences. They were tested in vitro to NaCl and temperature tolerance, auxin production and calcium phosphate solubilization. The symbiotic ability were assessed in an greenhouse experiment. A total of 32 bacteria were obtained and 17 amplified both symbiotic genes. The bacteria showed a high variable metabolic profile. Bradyrhizobium (6), Rhizobium (3) and Paraburkholderia (1) were identified, differing from their geographic origin. The isolates grew up to 45 °C to 0.51 mol L-1 of NaCl. Bacteria which produced more auxin in the medium with l-tryptophan and two Rhizobium and one Bradyrhizobium were phosphate solubilizers. All bacteria nodulated and ESA 90 (Rhizobium sp.) plus ESA 96 (Paraburkholderia sp.) were more efficient symbiotically. Diverse and efficient rhizobia inhabit the soils of Caatinga dry forests, with the bacterial differentiation by the sampling sites.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300503
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1016/j.bjm.2017.09.007
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.49 n.3 2018
Fonte: | info:eu-repo/semantics/openAccess