Data/hora: 10/06/2024 06:43:23

Provedor de dados: | 58
País: | Brazil
Título: | Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)
Autores: | Figueiredo,José Edson Fontes; Gomes,Eliane Aparecida; Guimarães,Claudia Teixeira; Lana,Ubiraci Gomes de Paula; Teixeira,Marta Aparecida; Lima,Guilherme Vitor Corrêa; Bressan,Wellington.
Data: | 2009-09-01
Ano: | 2009
Palavras-chave: | Endophytic bacteria; Bacillus; Sweet corn; SDS-PAGE; RDNA sequencing.
Resumo: | Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of wholecell protein extract of fortytwo isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing, Bacillus subitilis and B. pumilus were the most frequenty species (15 and 12 isolates, respectively) followed by B. licheniformes (7 isolates), B. cereus (5 isolates) and B. amiloliquefascens (3 isolates). According to present results, SDS-PAGE technique could be used as a fast and cheap first tool for identifying interspecific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intraspecific variation among isolates with similar protein profile as well as for taxonomic studies.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300014
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S1517-83822009000300014
Formato: | text/html
Fonte: | Brazilian Journal of Microbiology  v.40 n.3 2009
Fonte: | info:eu-repo/semantics/openAccess