Data/hora: 13/06/2024 01:02:49

Provedor de dados: 	58	
País: 	Brazil	
Título: 	Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans	
Autores: 	Nakanishi,Fernanda Akemi; Avila-Campos,Mario Julio; Kamiji,Nádia Hizuru; Itano,Eiko Nakagawa.	
Data: 	2006-03-01	
Ano: 	2006	
Palavras-chave: 	Immunoglobulin G; Actinobacillus actinomycetemcomitans; ELISA; Protease.	
Resumo: 	Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The molecular mass of the protease active fraction was from 43 to 150 kDa.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1590/S1517-83822006000100008	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.37 n.1 2006	
Fonte: 	info:eu-repo/semantics/openAccess