Data/hora: 13/06/2024 02:35:20

Provedor de dados: 	60	
País: 	Brazil	
Título: 	A sensitive bioanalytical method development and validation of cabozantinib in human plasma by LC-ESI-MS/MS	
Autores: 	Inturi,Srikanth; Avula,Prameela Rani.	
Data: 	2018-01-01	
Ano: 	2018	
Palavras-chave: 	Cabozantinib/human plasma/validation; Mass spectrometry; Pharmacokinetic/study.	
Resumo: 	ABSTRACT A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of cabozantinib (CZ) in human plasma using cabozantinib-d4 (CZD4) as an internal standard (IS). Chromatographic separation was performed on Xbridge C18, 50 x 4.6 mm, 5 mm column with an isocratic mobile phase composed of 10mM Ammonium formate and Methanol in the ratio of (20:80 v/v), at a flow-rate of 0.7 mL/min. CZ and CZD4 were detected with proton adducts at m/z 502.2 ® 391.1 and 506.3 ® 391.2 in multiple reaction monitoring (MRM) positive mode respectively. Liquid-Liquid extraction method was used to extract the drug and IS. The method was validated over a linear concentration range of 5.0-5000.0 pg/mL with correlation coefficient (r2) ≥ 0.9994. This method demonstrated intra and inter-day precision within 1.95 to 2.37 and 2.93 to 9.3 % and Accuracy within 101.4 to 102.4 and 99.5 to 104.8 %. Cabozantinib was found to be stable throughout freeze-thawing cycles, bench top and postoperative stability studies.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200622	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/s2175-97902018000217163	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.54 n.2 2018	
Fonte: 	info:eu-repo/semantics/openAccess