Data/hora: 11/06/2024 12:41:57

Provedor de dados: | 63
País: | Brazil
Título: | A vector carrying the GFP gene (Green fluorescent protein) as a yeast marker for fermentation processes
Autores: | Gomes,Luiz Humberto; Duarte,Keila Maria Roncato; Andrino,Felipe Gabriel; Giacomelli,Ana Maria Brancalion; Tavares,Flavio Cesar Almeida.
Data: | 2000-12-01
Ano: | 2000
Palavras-chave: | Saccharomyces cerevisiae; GFP (green fluorescent protein); Fermentative processes.
Resumo: | Contaminant yeasts spoil pure culture fermentations and cause great losses in quality and product yields. They can be detected by a variety of methods although none being so efficient for early detection of contaminant yeast cells that appear at low frequency. Pure cultures bearing genetic markers can ease the direct identification of cells and colonies among contaminants. Fast and easy detection are desired and morphological markers would even help the direct visualization of marked pure cultures among contaminants. The GFP gene for green fluorescent protein of Aquorea victoria, proved to be a very efficient marker to visualize transformed cells in mixed populations and tissues. To test this marker in the study of contaminated yeast fermentations, the GFP gene was used to construct a vector under the control of the ADH2 promoter (pYGFP3). Since ADH2 is repressed by glucose the expression of the protein would not interfere in the course of fermentation. The transformed yeasts with the vector pYGFP3 showed high stability and high bioluminescence to permit identification of marked cells among a mixed population of cells. The vector opens the possibility to conduct further studies aiming to develop an efficient method for early detection of spoilage yeasts in industrial fermentative processes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400018
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90162000000400018
Formato: | text/html
Fonte: | Scientia Agricola  v.57 n.4 2000
Fonte: | info:eu-repo/semantics/openAccess