Data/hora: 03/06/2024 11:11:25

Provedor de dados: | 63
País: | Brazil
Título: | Genetic variability in natural populations of Zeyheria montana mart. from the Brazilian Cerrado
Autores: | Bertoni,Bianca Waléria; Astolfi Filho,Spartaco; Martins,Ernani Ronie; Damião Filho,Carlos Ferreira; França,Suzelei de Castro; Pereira,Ana Maria Soares; Telles,Mariana Pires de Campos; Diniz Filho,José Alexandre F.
Data: | 2007-08-01
Ano: | 2007
Palavras-chave: | Bignoniaceae; Germplasm bank; Bolsa-de-pastor; Conservation.
Resumo: | Zeyheria montana, an endemic species of the Bignoniaceae family from the Brazilian Cerrado's known for its anti-cancer properties, is widely used as imuno stimulant in the popular medicine and its therapeutic activity must be validated by scientific data. The objective of this work was to evaluate the genetic variability of eight plant populations collected within the state of São Paulo, Brazil, via Random Amplification of Polymorphic DNA (RAPD) used as molecular markers. After an optimized protocol for the amplification reaction, nine selected primers generated 105 reproducible bands, indicating up to 60% polymorphism. Analysis of molecular variance (AMOVA) revealed higher genetic variation within populations (84.03%) than among populations (15.97%). The variation values estimated by phiST (0.160) indicated moderate to high inter population structuration. Levels of similarity inter plants with genetic and geographical distances, estimated by the unweighted pair-group method analysis (UPGMA) clustering and non-metric multidimensional scaling (NMDS) ordination methods and by the Mantel test (-0.2345 p = 0.118) denoted that the structure found follows the island model, which assumes that a single population of infinite size may have initiated the existing populations of Zeyheria montana, with no spatial position correlation. Based on the obtained data, a germplasm bank from individuals representing the species variability was established. Furthermore the information here reported can be of importance to develop strategies for the conservation of Z. montana.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000400012
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90162007000400012
Formato: | text/html
Fonte: | Scientia Agricola  v.64 n.4 2007
Fonte: | info:eu-repo/semantics/openAccess