Data/hora: 13/06/2024 04:22:04

Provedor de dados: | 63
País: | Brazil
Título: | Determination of aspartate kinase activity in maize tissues
Autores: | Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Gratão,Priscila Lupino; Lea,Peter John; Azevedo,Ricardo Antunes.
Data: | 2005-04-01
Ano: | 2005
Palavras-chave: | Aspartate pathway; Maize; Lysine; Threonine; Methionine.
Resumo: | Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90162005000200015
Formato: | text/html
Fonte: | Scientia Agricola  v.62 n.2 2005
Fonte: | info:eu-repo/semantics/openAccess